Proteomics

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Proteomic analysis of extracellular membrane vesicles from Pseudomonas aeruginosa (PaOMVs)

ABSTRACT: PaOMVs in the culture supernatant of P. aeruginosa were harvested by ultracentrifugation at 100,000 × g, 4℃ for 90 min using a Himac CP80WX Preparative Ultracentrifuge (HITACHI, Tokyo, Japan). After removal of the supernatant, the pellet was washed twice and suspended in ice-cold sterile phosphate-buffered saline (PBS). PaOMVs were then isolated from other damaged membranes, aggregated proteins and non-membranous proteins by step-gradient ultracentrifugation using OptiPrep™ Density Medium (Sigma Aldrich, St. Louis, MO). After centrifugation at 100,000 × g, 4℃ for 16 h, the substances in all six fractions (F1-F6) were collected, diluted in PBS, and harvested by ultracentrifugation at 100,000 × g, 4℃ for 3 h. The pellet from each fraction was washed twice and resuspended in a suitable volume of ice-cold sterile PBS.

ORGANISM(S): Pseudomonas Aeruginosa

SUBMITTER: Krisana Asano 

PROVIDER: PXD036377 | JPOST Repository | Tue Jul 18 00:00:00 BST 2023

REPOSITORIES: jPOST

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Publications

Extracellular vesicles of <i>Pseudomonas aeruginosa</i> downregulate pyruvate fermentation enzymes and inhibit the initial growth of <i>Staphylococcus aureus</i>.

Ishiai Takahito T   Subsomwong Phawinee P   Narita Kouj K   Kawai Noriaki N   Teng Wei W   Suzuki Sachio S   Sukchawalit Rojana R   Nakane Akio A   Asano Krisana K  

Current research in microbial sciences 20230420

<i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> are well-known opportunistic pathogens that frequently coexist in chronic wounds and cystic fibrosis. The exoproducts of <i>P. aeruginosa</i> have been shown to affect the growth and pathogenicity of <i>S. aureus,</i> but the detailed mechanisms are not well understood. In this study, we investigated the effect of extracellular vesicles from <i>P. aeruginosa</i> (PaEVs) on the growth of <i>S. aureus</i>. We found that PaEVs inhibited  ...[more]

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