Proteomics

Dataset Information

0

Proteomic analysis of extracellular membrane vesicles from Pseudomonas aeruginosa (PaOMVs)

ABSTRACT: PaOMVs in the culture supernatant of P. aeruginosa were harvested by ultracentrifugation at 100,000 × g, 4℃ for 90 min using a Himac CP80WX Preparative Ultracentrifuge (HITACHI, Tokyo, Japan). After removal of the supernatant, the pellet was washed twice and suspended in ice-cold sterile phosphate-buffered saline (PBS). PaOMVs were then isolated from other damaged membranes, aggregated proteins and non-membranous proteins by step-gradient ultracentrifugation using OptiPrep™ Density Medium (Sigma Aldrich, St. Louis, MO). After centrifugation at 100,000 × g, 4℃ for 16 h, the substances in all six fractions (F1-F6) were collected, diluted in PBS, and harvested by ultracentrifugation at 100,000 × g, 4℃ for 3 h. The pellet from each fraction was washed twice and resuspended in a suitable volume of ice-cold sterile PBS.

ORGANISM(S): Pseudomonas Aeruginosa

SUBMITTER: Krisana Asano 

PROVIDER: PXD036377 | JPOST Repository | Tue Jul 18 00:00:00 BST 2023

REPOSITORIES: jPOST

Json Xml

Dataset's files

Source:
Action DRS
201222_Pa-ex_IDA.group Other
201222_Pa-ex_IDA.wiff Wiff
201222_Pa-ex_IDA.wiff.scan Wiff
201222_Pa-ex_IDA__FDR.xlsx Xlsx
Items per page:
1 - 4 of 4
altmetric image

Publications

Extracellular vesicles of <i>Pseudomonas aeruginosa</i> downregulate pyruvate fermentation enzymes and inhibit the initial growth of <i>Staphylococcus aureus</i>.

Ishiai Takahito T   Subsomwong Phawinee P   Narita Kouj K   Kawai Noriaki N   Teng Wei W   Suzuki Sachio S   Sukchawalit Rojana R   Nakane Akio A   Asano Krisana K  

Current research in microbial sciences 20230420

<i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> are well-known opportunistic pathogens that frequently coexist in chronic wounds and cystic fibrosis. The exoproducts of <i>P. aeruginosa</i> have been shown to affect the growth and pathogenicity of <i>S. aureus,</i> but the detailed mechanisms are not well understood. In this study, we investigated the effect of extracellular vesicles from <i>P. aeruginosa</i> (PaEVs) on the growth of <i>S. aureus</i>. We found that PaEVs inhibited  ...[more]

Similar Datasets

Proteomics Quantitative proteomic analysis of extracellular membrane vesicles from Acinetobacter baumannii (AbOMVs) isolated under normoxic and hypoxic conditions.
2023-03-16 | PXD037014 | JPOST Repository
Proteomics The proteins identified from Staphylococcus aureus-derived extracellular vesicles (SaEVs)
2024-01-30 | PXD043854 | JPOST Repository
Other Real-time quantitative PCR analysis of Chromatin-associated snoRNA in human NB4 cells using Arraystar_384 platform
2021-05-04 | GSE173730 | GEO
Other m6A profiles in shCTRL, shMETTL3 HeLa cells
2019-10-15 | GSE134103 | GEO
Proteomics Yeast Heatshock SILAC LC-MSMS
2020-06-12 | PXD012631 | Pride
Proteomics Shewanella oneidensis outer membrane and outer membrane vesicles
2021-11-10 | PXD020577 | Pride
Proteomics Multifactorial remodeling of the cancer immunopeptidome by interferon gamma
2023-09-29 | PXD031634 | Pride
Transcriptomics Real-time quantitative PCR array analysis of primary colon cancer and liver metastasis derived exosomes
2017-01-04 | GSE93092 | GEO
Proteomics Ubiquitination in treated T. cruzi parasites
2022-02-17 | PXD027524 | Pride
Other ALKBH3 CLIP-seq in HeLa cells.
2022-03-01 | GSE112625 | GEO