Dataset Information

The proteins identified from Staphylococcus aureus-derived extracellular vesicles (SaEVs)

ABSTRACT: Staphylococcus aureus strain ATCC 1718 was inoculated at 0.5% in 400 mL of Brain Heart Infusion broth for 8 h under aerobic conditions. The supernatant was collected by centrifugation twice at 5,000 x g, 4°C for 20 min, filtrated through a 0.45 µm filter to remove all remaining bacterial cells. S. aureus-derived extracellular vesicles (SaEVs) in culture supernatant were precipitated by ultracentrifugation at 100,000 x g, 4°C for 90 min using a Himac CP80WX Preparative Ultracentrifuge (HITACHI, Tokyo, Japan). After washing with ice-cold PBS, the pellet was suspended and applied to discontinuous iodixanol gradient ultracentrifugation. Layers of 40%, 20%, 10%, and 5% iodixanol in 0.25 M sucrose/10 mM Tris, pH 7.5 were prepared using OptiPrep™ [60% (w/v) iodixanol; Sigma Aldrich, St. Louis, MO]. After centrifugation at 100,000 x g, 4°C for 16 h, the substances in all six fractions (Fr1-Fr6) were collected, diluted in PBS, and precipitated by ultracentrifugation at 100,000 × g, 4°C for 2 h. The pellets were then washed and resuspended in an appropriate volume of ice-cold PBS.

ORGANISM(S): Staphylococcus Aureus

SUBMITTER: Krisana Asano

PROVIDER: PXD043854 | JPOST Repository | Tue Jan 30 00:00:00 GMT 2024

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	220614_SaEVs_MSSA_Fr5_IDA.1718.group	Other
	220614_SaEVs_MSSA_Fr5_IDA.1718__FDR.xlsx	Xlsx
	220614_SaEVs_MSSA_Fr5_IDA.wiff	Wiff
	220614_SaEVs_MSSA_Fr5_IDA.wiff.scan	Wiff

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Publications

Extracellular vesicles from Staphylococcus aureus promote the pathogenicity of Pseudomonas aeruginosa.

Subsomwong Phawinee P Teng Wei W Ishiai Takahito T Narita Kouji K Sukchawalit Rojana R Nakane Akio A Asano Krisana K

Microbiological research 20240117

Co-infections with Staphylococcus aureus and Pseudomonas aeruginosa are common in patients with chronic wounds, but little is known about their synergistic effect mediated by extracellular vesicles (EVs). In this study, we investigated the effect of EVs derived from S. aureus (SaEVs) on the pathogenicity of P. aeruginosa. By using lipophilic dye, we could confirm the fusion between SaEV and P. aeruginosa membranes. However, SaEVs did not alter the growth and antibiotic susceptible pattern of P. ...[more]

PMID: 38244256

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