Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Utilizing MALDI-TOF mass spectrometry and liquid chromatography-tandem mass spectrometry to access serum peptidome-based biomarkers in canine oral tumors

ABSTRACT: Tumors frequently found in dogs include canine oral tumors, either cancerous or noncancerous. The bloodstream is an important route for tumor metastasis, particularly for late-stage oral melanoma (LOM) and late-stage oral squamous cell carcinoma (LOSCC). The present study aimed to investigate serum peptidome-based biomarkers of dogs with early-stage oral melanoma, LOM, LOSCC, benign oral tumors, chronic periodontitis and healthy controls, using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and liquid chromatography tandem mass spectrometry.

ORGANISM(S): Cellular Organisms

SUBMITTER: Sittiruk Roytrakul

PROVIDER: PXD037051 | JPOST Repository | Thu Sep 28 00:00:00 BST 2023

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ABCB5.pdf	Pdf
	CE_26%2525_RC6_01_59990.mgf	Mgf
	ESRRA.pdf	Pdf
	FKBP4.pdf	Pdf
	IGSF10.pdf	Pdf

Items per page:

1 - 5 of 10

Similar Datasets

Quantitative Label Free Ultra Definition Mass-Spectrometry Provides Novel Data on the Proteomic Signature of Oral Squamous Cell Carcinoma

Project description:There are no early detection biomarkers or prognostic markers for oral squamous cell carcinoma (OSCC) thus many are detected late, with unpredictable disease course. Using systems level analysis on shotgun proteomics with quantitative label-free ultra-definition mass spectrometry, our study aims to find novel proteins involved in both low and high grade OSCC tissue, and to further understand pathways involved in cancer development and progression, using proteomic analysis.

2022-03-02 | PXD009244 | Pride

Apolipoprotein E promotes invasion in oral squamous cell carcinoma

Project description:Genome-wide expression array measurements for 9 head and neck squamous cell carcinomas (HNSCC) stratified by worst pattern of invasion (WPOI) Jayakar et al. (2016). Apolipoprotein E promotes invasion in oral squamous cell carcinoma. Li et al. (2013). Validation of the risk model: high-risk classification and tumor pattern of invasion predict outcome for patients with low-stage oral cavity squamous cell carcinoma. Comparison of transcription profiles between OSCC tumors with a more invasive (WPOI 5) versus a less invasive (WPOI 3) pattern of invasion using two independent Illumina platforms.

2016-04-01 | E-GEOD-79795 | biostudies-arrayexpress

Integrative MHC-I peptidome quantitation with isobaric tagging mass spectrometry

Project description:Despite the appreciation of CD8+ T cell anti-tumor immune responses towards improvement patient outcomes, the MHC-I peptides that facilitate the response are poorly described. Alternatively, whether cancer therapies alter the MHC-I peptide repertoire has not been fully assessed due to the lack of quantitative strategies. In this regard, anthracyclines and ionizing radiation both increase the infiltration of CD8+ T cells into tumors but whether they do so by altering the MHC-I peptidome repertoire is not known. To investigate this, we developed a platform for screening therapy-induced MHC-I peptides by quantitative, multiplexed measurement of MHC-I peptides with tandem mass tags (TMT). We show that both ionizing radiation and the anthracycline doxorubicin induce MHC-I peptides that are largely derived from mitotic progression and cell cycle proteins. Our approach enables further strategies to understand how small molecules and other therapies alter MHC-I peptide presentation that may be harnessed for CD8+ T cell-based immunotherapies.

2019-03-05 | PXD011464 | Pride

Integrative MHC-I peptidome quantitation with isobaric tagging mass spectrometry

2019-03-05 | PXD011444 | Pride

MHC-II peptidome of the CNS reveals endogenous guardian peptides

Project description:MHC-II peptidome of the CNS reveals endogenous guardian peptides

| PRJNA1004626 | ENA

Characterization of resistance to tumor-localized interleukin-2 and interleukin-12 therapy in pet dogs with advanced malignant melanoma

Project description:Pet dogs with oral melanoma were treated in a dose-escalation trial with collagen-anchored interleukin-2 and interleukin-12 following a single 9Gy dose of radiation therapy. Dogs that progressed during, or after, treatment and were ultimately euthanized had tissue collected from primary and metastatic tumors to analyze gene expression and assess mechanisms of resistance to this therapy.

2024-01-29 | GSE253243 | GEO

Mass spectrometry-based lipidomics of oral squamous cell carcinoma tissue reveals aberrant cholesterol and glycerophospholipid metabolism

Project description:Comparison between the lipid profile in oral squamous cell carcinoma of the tongue and healthy epithelial tissue from the contralateral side of the tongue of the same patient.

2019-10-09 | ST001267 | MetabolomicsWorkbench

Quantification of microRNA expression in early-stage oral squamous cell carcinoma

Project description:Microarray was used to find out the differentially expressed in tumor sites of early-stage oral squamous cell carcinoma compared with Normal parts. Furthermore, we compared cases of early-stage oral squamous cell carcinoma with lymph node metastasis with cases without lymph node metastasis. The miRNAs obtained may not only serve as predictive biomarkers for lymph node metastasis, but may also be used further to understand disease.

2023-08-22 | GSE241289 | GEO

MMP qualitative and quantitative mass spectrometry

Project description:Methanococcus maripaludis is a methanogenic archaeon. Within its genome, there are two operons for membrane associated hydrogenases, eha and ehb. To investigate the regulation of ehb on the cell, an S40 mutant was constructed in such a way that a portion of the ehb operon was replaced by pac cassette in the wild type parental strain S2 (done by Whitman's group at the University of Georgia). The S40 and S2 strains were grown in 14N and 15N media with acetate separately. A biological replicate was made by switching the media. Mass spectrometry based quantitative proteomics were done on the mixtures to investigate the differences in expression patterns between S40 and S2. Keywords: isotope labeling mass spectrometry, quantitative proteomics

2005-09-20 | GSE2744 | GEO

OSCC whole genome expression by T stage

Project description:Differentially expressed genes between T4 and T1 stage human oral squamous cell carcinoma (OSCC)

2015-06-23 | GSE59069 | GEO