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A multiplexed microflow LC-MS/MS-PRM assay for serologic quantification of IgG N- and HPX O- glycoforms in liver fibrosis

ABSTRACT: In this study, we introduce a targeted microflow LC-MS/MS-PRM method for the quantification of multiple glycopeptides in unfractionated serum samples. The entire preparation of 16 samples in a batch is completed within 3 h, and the LC-MS quantification of all the glycoforms in a sample is completed in 15 min in triplicate, including online capture and desalting. We demonstrate applicability of the workflow on a multiplexed quantification of eight N-glycoforms of immunoglobulin G (IgG) together with two O-glycoforms of hemopexin (HPX). We applied the assay to a serologic study of fibrotic liver disease in patients of HCV etiology. The results document that specific IgG- and HPX-glycoforms detect efficiently fibrotic disease of different degree, and suggest that the LC-MS/MS-PRM assays may provide rapid and reproducible biomarker assay targeting simultaneously the N- and O-glycoforms of the peptides.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Radoslav Goldman

PROVIDER: PXD037687 | JPOST Repository | Wed Oct 25 00:00:00 BST 2023

REPOSITORIES: jPOST

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			Action	DRS
	062322-PRM-S01-1.raw	Raw
	062322-PRM-S01-2.raw	Raw
	062322-PRM-S01-3.raw	Raw
	062322-PRM-S02-1.raw	Raw
	062322-PRM-S02-2.raw	Raw

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