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Investigating the role of METTL8 in sustaining GBM hallmarks

ABSTRACT: Methyltransferase-like 8 (METTL8) is a RNA methyltransferase that plays a crucial role in mitochondrial translation. Previous study reported that METTL8 is overexpressed in cancer, but whether it contributes to GBM pathogenesis has not been explored. Here, we find that METTL8 is overexpressed in GBM stem cells (GSC) relative to non-cancerous human neural progenitor cells and astrocytes. METTL8 depletion significantly reduces GSC tumorsphere formation, differentiation potential, invasiveness and tumorigenicity. As expected, METTL8 is localized to the mitochondrial matrix of GSC. Although it is established that METTL8 binds to tRNAs, the protein interactors of METTL8 remains uncharacterized. To this end, we aim to define the METTL8 interactome using mitochondrial fractions of 293T cells, and then validate the identified interactors in GSC.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Derrick Ong Sek Tong

PROVIDER: PXD041018 | JPOST Repository | Fri Mar 22 00:00:00 GMT 2024

REPOSITORIES: jPOST

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Dataset's files

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			Action	DRS
	20190611_Human_UniProt.fasta	Fasta
	20211009_JW_Bernice_METTL8_7500_TMT6_20ppm.msf	Msf
	20211009_JW_Bernice_METTL8_7500_TMT6_20ppm.pdResult	Other
	20211009_JW_Bernice_METTL8_7500_TMT6_20ppm.xlsx	Xlsx
	20211009_JW_Bernice_METTL8_TMT6_F1.raw	Raw

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