Dataset Information

Human AC16 Cardiac Cells under Doxorubicin Treatment

ABSTRACT: AC16 acquired from Millipore and cultured in DMEM/F12 with 10% FBS. For the mass spectrometry experiment, the AC16 cells were switched to Fibroblast Growth Medium (FGM-3) with supplements (Promocell) then treated with 0.1 µM doxorubicin for 24 hours, then co-cultured through non-contact transwells with primary human ventricular fibroblasts (Promocell) with or without TGFb stimulation for 24 hours. The induction of senescence with doxorubicin and lack of spontaneous senescence was verified using p21 immunoblots. For mass spectrometry, 25 µg of proteins were reduced, alkylated, and digested with trypsin using a filter-assisted protocol on a Pierce 10 K MWCO column. The peptides were then labeled using Tandem Mass Tag (TMT) 10-plex reagents (Thermo), combined, and separated by high-pH reversed phase separation (Thermo), then analyzed on an Orbitrap HF mass spectrometer.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Edward Lau

PROVIDER: PXD041722 | JPOST Repository | Sat Apr 22 00:00:00 BST 2023

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	20220919_VH_AC16_CoCulture_TMT_F1.mzML.gz	Mzml
	20220919_VH_AC16_CoCulture_TMT_F1.pep.xml	Pepxml
	20220919_VH_AC16_CoCulture_TMT_F1.raw	Raw
	20220919_VH_AC16_CoCulture_TMT_F2.mzML.gz	Mzml
	20220919_VH_AC16_CoCulture_TMT_F2.pep.xml	Pepxml

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Publications

Senescence in human AC16 cardiac cells is associated with thymidine kinase induction and histone loss.

Kastury Nikhitha N Hidalgo Veronica V Pandi Boomathi B Li Lauren L Lam Maggie P Y MPY Lau Edward E

microPublication biology 20230629

AC16 cells are a transformed human cardiac cell line commonly used to study cardiomyocyte biology. We show that reduced proliferation and senescence markers can be robustly induced in AC16 cells cultured in low serum condition and treated with (i) low-dose doxorubicin, (ii) UV 254 nm, or (iii) H <sub>2</sub> O <sub>2</sub> exposure for up to 48 hours. Increased p21 (CDKN1A) and H2A.X variant histone (H2AX) levels serve as reliable molecular markers upon all three treatment conditions, but the up ...[more]

PMID: 37456137

Dataset Information

Human AC16 Cardiac Cells under Doxorubicin Treatment

Dataset's files

Publications

Senescence in human AC16 cardiac cells is associated with thymidine kinase induction and histone loss.

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