Proteomics

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Identification of gravity-responsive proteins in the femur of spaceflight mice using a quantitative proteomic approach

ABSTRACT: This project is “Medical proteome analysis of osteoporosis- and bone mass-related proteins using the Kibo Japanese Experiment Module of International Space Station (ISS)”. Utilizing proteomic technologies, this research aims to identify proteins involved in space flight-induced osteopenia as well as osteoporosis occurring on the ground by searching serum, and skeletal muscle and bone (femoral diaphysis, mandible) proteomes. The goal of this study is to get better understanding to protect the health of both astronauts and osteoporosis patients. Proteome analyses of C57BL/6J space flown mouse (micro-G and artificial 1-G [n = 5/group, in femoral diaphysis and n = 6/group in mandible]) were carried out tryptic digestion followed by LC-MS/MS analysis (DIA). The obtained results were analyzed using Spectronaut Pulsar X against the mouse-bone spectral library for identification and quantification of proteins.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Yayoi Kimura 

PROVIDER: PXD041738 | JPOST Repository | Tue Aug 01 00:00:00 BST 2023

REPOSITORIES: jPOST

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180419QE_SpaceMouse_bone_1.raw Raw
180419QE_SpaceMouse_bone_11.raw Raw
180419QE_SpaceMouse_bone_12.raw Raw
180419QE_SpaceMouse_bone_2.raw Raw
180419QE_SpaceMouse_bone_3.raw Raw
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Identification of gravity-responsive proteins in the femur of spaceflight mice using a quantitative proteomic approach.

Egashira Kenji K   Ino Yoko Y   Nakai Yusuke Y   Ohira Takashi T   Akiyama Tomoko T   Moriyama Kayano K   Yamamoto Yu Y   Kimura Mitsuo M   Ryo Akihide A   Saito Tomoyuki T   Inaba Yutaka Y   Hirano Hisashi H   Kumagai Ken K   Kimura Yayoi Y  

Journal of proteomics 20230722

Although the microgravity (μ-g) environment that astronauts encounter during spaceflight can cause severe acute bone loss, the molecular mechanism of this bone loss remains unclear. To investigate the gravity-response proteins involved in bone metabolism, it is important to comprehensively determine which proteins exhibit differential abundance associated with mechanical stimuli. However, comprehensive proteomic analysis using small bone samples is difficult because protein extraction in mineral  ...[more]

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