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SK_16: Proteomic analysis of the effects of plant-derived smoke in wheat under salt stress

ABSTRACT: Proteomic analysis of the effects of plant-derived smoke in wheat under salt stress.

ORGANISM(S): Triticum Aestivum

SUBMITTER: Setsuko Komatsu

PROVIDER: PXD043228 | JPOST Repository | Thu Aug 29 00:00:00 BST 2024

REPOSITORIES: jPOST

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			Action	DRS
	20230615_09_WheatRoot_C1_130m1uL.raw	Raw
	20230615_12_WheatRoot_C2_130m1uL.raw	Raw
	20230615_15_WheatRoot_C3_130m1uL.raw	Raw
	20230615_18_WheatRoot_S1_130m1uL.raw	Raw
	20230615_21_WheatRoot_S2_130m1uL.raw	Raw

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Publications

Metabolomic and Proteomic Analyses to Reveal the Role of Plant-Derived Smoke Solution on Wheat under Salt Stress.

Komatsu Setsuko S Diniyah Azzahrah A Zhu Wei W Nakano Masataka M Rehman Shafiq Ur SU Yamaguchi Hisateru H Hitachi Keisuke K Tsuchida Kunihiro K

International journal of molecular sciences 20240727 15

Salt stress is a serious problem, because it reduces the plant growth and seed yield of wheat. To investigate the salt-tolerant mechanism of wheat caused by plant-derived smoke (PDS) solution, metabolomic and proteomic techniques were used. PDS solution, which repairs the growth inhibition of wheat under salt stress, contains metabolites related to flavonoid biosynthesis. Wheat was treated with PDS solution under salt stress and proteins were analyzed using a gel-free/label-free proteomic techni ...[more]

PMID: 39125784

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Expression profile in response to salt-treatment in wheat using 11k wheat array

Project description:Global expression analysis of transcripts in response to salt treatment was carried out for common wheat using oligo-DNA microarrays. Microarrays have been designed from unique wheat genes classified from a large number of expressed sequence tags (ESTs). Two-week-old seedlings of common wheat were treated with 150 mM NaCl for 1, 6 and 24 hours and their roots and shoots were separately subjected to microarray analyses. Consequently, 5996 genes showed changes in expression of more than two-fold, and were classified into 12 groups according to correlations in gene expression patterns. These salt-responsive genes were assigned functions using Gene Ontology (GO) terms. Genes assigned to transcription factor, transcription-regulator activity and DNA binding functions were preferentially classified into early response groups. On the other hand, those assigned transferase and transporter activity were classified into late response groups. These data on gene expression suggest that multiple signal transduction pathways in response to salt treatment exist in wheat. Salt-responsive transcription factors (TFs), namely AP2/EREBP, MYB, NAC and WRKY, were selected and their expression patterns compared with those of rice. Most showed different expression patterns in wheat and rice in response to salt treatment. Furthermore, comparing the microarray data for wheat and rice, only a small number of genes were up- or down-regulated in common in response to salt treatment. These findings suggest that salt-responsive mechanisms distinct from rice might be present in wheat, and wheat genes can contribute to providing novel gene resources for breeding of salt-tolerant crops. Keywords: time cource, stress response

2007-10-02 | GSE8064 | GEO

Expression profile in response to salt-treatment in wheat using 22k microarray

Project description:Analysis of transcripts in response to salt treatment. In order to design the 22k wheat oligo-DNA microarray, a total of 148,676 expressed sequence tags of common wheat were collected from the database of the Wheat Genomics Consortium of Japan. These were grouped into 34,064 contigs, which were then used to design an oligonucleotide DNA microarray. Following a multi-step selection of the sense strand, 21,939 60-mer oligo DNA probes were selected for attachment on the microarray slide. This 22k oligo DNA microarray was used to examine the transcriptional response of wheat to salt stress. More than 95% of the probes gave reproducible hybridization signals when targeted with RNAs extracted from salt-treated wheat shoots and roots. With the microarray, we identified 1,811 genes whose expressions changed more than two-fold in response to salt. These included genes known to mediate the response to salt as well as unknown genes, and they were classified into 12 major groups by hierarchical clustering. These gene expression patterns were also confirmed by real-time reverse transcription (RT)-PCR. Many of the genes with unknown function were clustered together with genes known to be involved in the response to salt stress. Thus, analysis of gene expression patterns combined with gene ontology should help identify the function of the unknown genes. Also, functional analysis of these wheat genes should provide new insight into the response to salt stress. Finally, these results indicate that the 22k oligo DNA microarray is a reliable method for monitoring global gene expression patterns in wheat. Keywords: time cource, stress response

2007-10-02 | GSE8060 | GEO

Expression profile in response to salt-treatment in wheat using 11k wheat array

2007-10-02 | E-GEOD-8064 | biostudies-arrayexpress

Expression profile in response to salt-treatment in wheat

Project description:This SuperSeries is composed of the following subset Series: GSE8060: Expression profile in response to salt-treatment in wheat using 22k microarray GSE8064: Expression profile in response to salt-treatment in wheat using 11k wheat array Refer to individual Series

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Project description:An TMT-based quantitative crotonylome analysis was performed on the crotonylated proteins enriched from the chloroplast extracts of the normal and salt-treated wheat seedling leaves by using affinity purification and LC-MS/MS. The crotonylated peptides from wheat chloroplast extracts were enriched by using anti-crotonyllysine mouse mAb (Clone 4D5) antibody (PTM BioLabs, HangZhou, China PTM-501 and Nano LC-MS/MS was performed by using a Dionex rapid-separation liquid chromatography system interfaced with a Q Exactive HF (Thermo Fisher Scientific).

2023-01-18 | PXD033662 | Pride

Metabolomic and Proteomic Analyses to Reveal the Role of Plant-Derived Smoke Solution on Wheat under Salt Stress.

Project description:Salt stress is a serious problem, because it reduces the plant growth and seed yield of wheat. To investigate the salt-tolerant mechanism of wheat caused by plant-derived smoke (PDS) solution, metabolomic and proteomic techniques were used. PDS solution, which repairs the growth inhibition of wheat under salt stress, contains metabolites related to flavonoid biosynthesis. Wheat was treated with PDS solution under salt stress and proteins were analyzed using a gel-free/label-free proteomic technique. Oppositely changed proteins were associated with protein metabolism and signal transduction in biological processes, as well as mitochondrion, endoplasmic reticulum/Golgi, and plasma membrane in cellular components with PDS solution under salt stress compared to control. Using immuno-blot analysis, proteomic results confirmed that ascorbate peroxidase increased with salt stress and decreased with additional PDS solution; however, H+-ATPase displayed opposite effects. Ubiquitin increased with salt stress and decreased with additional PDS solution; nevertheless, genomic DNA did not change. As part of mitochondrion-related events, the contents of ATP increased with salt stress and recovered with additional PDS solution. These results suggest that PDS solution enhances wheat growth suppressed by salt stress through the regulation of energy metabolism and the ubiquitin-proteasome system related to flavonoid metabolism.

| S-EPMC11311447 | biostudies-literature

Expression profile in response to salt-treatment in wheat using 22k microarray

2007-10-02 | E-GEOD-8060 | biostudies-arrayexpress

Integrated Physiological and Large-Scale Chloroplast Proteome Analysis of Wheat Seedling Leaves under Salt and PEG-Induced Drought Stresses

Project description:The integrative physiological and large-scale quantitative proteomic analysis of wheat chloroplast under salt and polyethylene glycol (PEG)-induced drought stresses by using label-free quantitative technique. We aim to reveal the underlying synergistic responsive mechanisms of wheat chloroplast proteome to salt and osmotic stresses, which could provide a more in-depth and comprehensive understanding on the roles of chloroplast proteome in abiotic stress response and defense.

2021-09-09 | PXD019453 | Pride

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