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Conditioned media from menstrual blood‑derived stem cells

ABSTRACT: We collected menstrual blood-derived cells from a volunteer and Asherman's syndrome, and confirmed paracrine capacities of these cells.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Satoshi Hosoya

PROVIDER: PXD045424 | JPOST Repository | Tue Sep 17 00:00:00 BST 2024

REPOSITORIES: jPOST

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			Action	DRS
	3%20Samples_SwissProt_Homo%20sapiens.sf3	Other
	INFERTILE-CM%20%231.mgf	Mgf
	INFERTILE-CM%20%231.mzid	Mzid
	INFERTILE-CM%20%231.raw	Raw
	M199-CM.mgf	Mgf

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Conditioned media from menstrual blood‑derived stem cells

Project description:We collected menstrual blood-derived cells from a volunteer and Asherman's syndrome, and confirmed paracrine capacities of these cells.

2024-09-17 | PXD044907 | JPOST Repository

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Gene expression analysis of Human CD34+ cells derived from bone marrow, mobilized peripheral blood, umbillial cord blood using Agilent Whole Human Genome Oligo Microarrays (one-color)

Project description:In order to identify genes associated with the engraftment potential of human hematopoietic stem cells, we have employed whole genome microarray expression profiling of G0 and G1 phase CD34+ cells derived from bone marrow, mobilized peripheral blood, and umbilical cord blood. Samples were collected from healthy adult volunteers after obtaining informed consent according to the guidelines of the Investigational Review Board of Indiana University School of Medicine. CD34+ cells were selected and fractionated into G0 and G1 phases of cell cycle on a flow cytometer. Purity of sorted cells was further confirmed by qRT-PCR by measuring the relative expression of Ki67. Sorted cells were subjeccted to microarray analysis. Three biological replicates of sorted and confirmed G0 and G1 cells from bone marrow, mobilized peripheral blood, and umbilical cord blood (total of eighteen samples) were subjected to microarray analysis. To generate distinct and unique sets of data, we did not pool multiple samples from any tissue studied so that each sample or its replicate was from a single donor.

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