ABSTRACT: Mouse retinal proteome for LFQ. The retinal samples of CCT2-LCA model of R516H/R516H (RH/RH) knock-in mice and its wild type littermates (WT/WT) were analyzed for LFQ.
Project description:Transcriptome of 8 and 18 weeks old mice where fibrosis developed upon the full-body knocking-out gene Glmp (glycosylated lysosomal membrane protein) (Glmpgt/gt; Glmp KO) Glmp KO mice were compared to their wild-type (WT) littermates
Project description:Ventricular tissue of 8 week female PA mice (https://pubmed.ncbi.nlm.nih.gov/23648696/) and their female wild-type littermates were processed for phosphoproteomics
Project description:Frataxin deficiency in human is the cause of Friedreich's ataxia (FA), a lethal neuro- and cardio-degenerative disease. Knock-out (KO) mice of this mouse model of FA exhibit classical cardiomyopathy of the patients. The onset of FA phenotypes in the KO mice is approximately 6-7 weeks of age. This genearray analysis was conducted to examine the changes in gene expression in the heart of KO mice relative to their wild-type (WT) littermates at 4- and 10-weeks of age. At 10-weeks of age, the KO mice begin to die from severe cardiomyopathy. RNA from the heart of four female 4-week-old MCK littermates (two WT and two KO) and four female 10-week-old MCK littermates (two WT and two KO) was extracted and hybridised to Affymetrix Mouse Genome 430 2.0 Array.
Project description:Mutations in COL6A3 lead to collagen VI-related myopathies due to COL6A3 loss. Therefore, studying the consequence of Col6a3 deregulated expression in mouse models is relevant, but the Col6a3 knockout mouse models currently available through a public repository does not entirely abolish COL6A3 protein expression. Here, we present the validation and phenotypic characterization of a novel CRISPR-based knockout mouse model targeting Col6a3 exon 3 (Col6a3 d3/d3). In this mouse model, Col6a3 mRNA is still expressed at a similar level to that of wild-type littermates, although the expected protein is undetectable. Histological analysis of Col6a3 d3/d3 quadriceps revealed an abnormally high frequency of muscle cells with a centralized nuclei, consistent with a muscular dystrophy phenotype. Interestingly, Col6a3 d3/d3 mice are smaller in size with their fat, muscle, and bone to body ratios kept proportional compared to wild type littermates. Thus, we developed a novel Col6a3 knockout mouse model that could be further used to study Col6a3 biology and collagen VI-associated diseases.
Project description:Frataxin deficiency in human is the cause of Friedreich's ataxia (FA), a lethal neuro- and cardio-degenerative disease. Knock-out (KO) mice of this mouse model of FA exhibit classical cardiomyopathy of the patients. The onset of FA phenotypes in the KO mice is approximately 6-7 weeks of age. This genearray analysis was conducted to examine the changes in gene expression in the heart of KO mice relative to their wild-type (WT) littermates at 4- and 10-weeks of age. At 10-weeks of age, the KO mice begin to die from severe cardiomyopathy.
Project description:Gene expression data from CD22+B220+ FACS-purified splenocytes of adult Sca1-HGAL knock-in CBAxC57BL/6J mice or wild-type littermates.
Project description:Olfaction is often deregulated in Alzheimer´s disease (AD) patients, being also impaired in transgenic Tg2576 AD mouse model, which overexpress the Swedish mutated form of human amyloid precursor protein (APP). However, little is known about the molecular mechanisms that accompany the neurodegeneration of olfactory structures in Tg2576 mice. For that, we have applied proteome- and transcriptome-wide approaches to probe molecular disturbances in the olfactory bulb (OB) dissected from aged Tg2576 mice (18 months of age) respect to age matched wild-type (WT) littermates.
Project description:Villin-TLR4 mice and their wild-type littermates underwent the AOM-DSS model of tumorigenesis and their non-involved tissues were analyzed for transcriptomic expression
Project description:Gene expression from livers of knock-out, hemizygous and wild-type mice at the age of , 8 and 12 months. Including tumor and non-tumor tissue of the same animals. AlbCre negative littermates were used as wild type controls.