Proteomics

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Formyl-methionine as an early elongational ribosome destabilization signal


ABSTRACT: Despite recent findings of cytosolic fMet-protein translation in eukaryotes, the underlying mechanism that limits cytosolic fMet-protein translation remains unknown. Here, we found that fMet-nascent peptide signals premature ribosome dissociation in S. cerevisiae, that we named N-terminal fMet-initiated ribosome quality control (fMet-RQC). pBpa-dependent photo-crosslinking of stalled fMet-nascent peptide co-purified with Nip1 and Arf1/2, suggesting their functional importance in fMet-RQC pathway. Defective fMet-RQC leads to co-translational fMet-protein aggregation hindering cellular stress adaptiveness. We also found that dynamics of fMet-RQC is critical in regulating cytosolic fMet-protein level during extreme environmental stresses, such as prolonged cold stress, for cellular survival.

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Cheol-Sang Hwang 

PROVIDER: PXD046345 | JPOST Repository | Thu Oct 24 00:00:00 BST 2024

REPOSITORIES: jPOST

Dataset's files

Source:
Action DRS
0531_ARF1.raw Raw
0531_ARF2.raw Raw
0531_ARF3.raw Raw
0531_WT1.raw Raw
20220517%20XL_LFQ_LAR_UNIPROT.mgf Mgf
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