Dataset Information

Proteome analysis of secretory substances containing proteins, extracellular vehicles, and others secreted from Candida albicans cultured under normal and hypoxic conditions.

ABSTRACT: Candida albicans were cultured under normal and hypoxic conditions and collected secretome from the culture supernatant with centrifugation. The secretome contains secretory proteins, extracellular vehicles, and others.

ORGANISM(S): Candida Albicans

SUBMITTER: Krisana Asano

PROVIDER: PXD046763 | JPOST Repository | Mon Jan 29 00:00:00 GMT 2024

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	211119_Asano_Hypo-Evs-Ca_210626_SWATH.wiff	Wiff
	211119_Asano_Hypo-Evs-Ca_210626_SWATH.wiff.scan	Wiff
	211119_Asano_Hypo-Evs-Ca_210909-1_SWATH.wiff	Wiff
	211119_Asano_Hypo-Evs-Ca_210909-1_SWATH.wiff.scan	Wiff
	211119_Asano_Hypo-Evs-Ca_210909-2_SWATH.wiff	Wiff

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Publications

Heat Shock Protein SSA1 Enriched in Hypoxic Secretome of Candida albicans Exerts an Immunomodulatory Effect via Regulating Macrophage Function.

Teng Wei W Subsomwong Phawinee P Narita Kouji K Nakane Akio A Asano Krisana K

Cells 20240110 2

Candida albicans is an opportunistic pathogenic yeast that can survive in both normoxic and hypoxic environments. The involvement of C. albicans secretome on host biological processes has been demonstrated. However, the immunoregulatory function of C. albicans secretome released under hypoxic condition remains unclear. This study demonstrated the differences in cytokine responses and protein profiles between secretomes prepared under normoxic and hypoxic conditions. Furtherm ...[more]

PMID: 38247818

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Project description:Background:Mesenchymal stem cells (MSCs) can be easily expanded. They can be acquired from medical waste such as adipose and umbilical cord tissues, are influenced by culturing conditions, and exert anti-inflammatory, antioxidant, anti-fibrotic, and angiogenic effects. We analyzed the multi-directional effects of MSCs cultured under hypoxic conditions and their underlying mechanisms in the treatment of liver cirrhosis in a mouse model. Methods:Human bone marrow-derived MSCs cultured under hypoxic (5% O2; hypoMSCs) and normoxic (21% O2; norMSCs) conditions were compared by cap analysis of gene expression (CAGE) with or without serum from liver cirrhosis patients. The therapeutic effects of MSCs, including serum liver enzyme induction, fibrosis regression, and hepatic oxidative stress, were evaluated by injecting 1 × 106, 2 × 105, or 4 × 104 MSCs/mouse into the tail veins of mice with carbon tetrachloride (CCl4)-induced liver cirrhosis. Intravital imaging was performed with a two-photon excitation microscope to confirm the various MSC migration paths to the liver. Results:CAGE analysis revealed that the RNA expression levels of prostaglandin E synthase (Ptges) and miR210 were significantly higher in hypoMSCs than in norMSCs. In vivo analysis revealed that both hypoMSCs and norMSCs reduced serum alanine aminotransferase, oxidative stress, and fibrosis compared to that in control mice in a dose-dependent manner. However, hypoMSCs had stronger therapeutic effects than norMSCs. We confirmed this observation by an in vitro study in which hypoMSCs changed macrophage polarity to an anti-inflammatory phenotype via prostaglandin E2 (PGE2) stimulation. In addition, miR210 reduced the rate of hepatocyte apoptosis. Intravital imaging after MSC administration showed that both cell types were primarily trapped in the lungs. Relatively a few hypoMSCs and norMSCs migrated to the liver. There were no significant differences in their distributions. Conclusion:The therapeutic effect of hypoMSCs was mediated by PGE2 and miR210 production and was greater than that of norMSCs. Therefore, MSCs can be manipulated to improve their therapeutic efficacy in the treatment of liver cirrhosis and could potentially serve in effective cell therapy. MSCs produce several factors with multidirectional effects and function as "conducting cells" in liver cirrhosis.

Dataset Information

Proteome analysis of secretory substances containing proteins, extracellular vehicles, and others secreted from Candida albicans cultured under normal and hypoxic conditions.

Dataset's files

Publications

Heat Shock Protein SSA1 Enriched in Hypoxic Secretome of <i>Candida albicans</i> Exerts an Immunomodulatory Effect via Regulating Macrophage Function.

Similar Datasets

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