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Total proteome and phosphoproteome of NIL-DOG1 and dog1-1 dry and 6 hours imbibed seed

ABSTRACT: We performed a label-free quantitative mass spectrometry-based proteomic analysis comparing the dry and imbibed seed proteome and phosphoproteome of two genotypes with contrasted level of dormancy in three independent biological replicates. This assay aimed to identified protein accumulation pattern and change in phosphopeptide abundances correlating with germination phenotypes.

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: IRIS FINKEMEIER

PROVIDER: PXD046985 | JPOST Repository | Fri Nov 15 00:00:00 GMT 2024

REPOSITORIES: jPOST

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			Action	DRS
	150924_150521_WS_GuNe_FLTisdbQ_01-1.raw	Raw
	150924_150521_WS_GuNe_FLTisdbQ_01-2.raw	Raw
	150924_150521_WS_GuNe_FLTisdbQ_01-3.raw	Raw
	150924_150521_WS_GuNe_FLTisdbQ_02-1.raw	Raw
	150924_150521_WS_GuNe_FLTisdbQ_02-2.raw	Raw

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Project description:Plant embryos can survive years in a desiccated, quiescent state within seeds. In many species, seeds are dormant and unable to germinate at maturity. They acquire the capacity to germinate through a period of dry storage called after-ripening (AR), a biological process that occurs at 5-15% moisture when most metabolic processes cease. Because stored transcripts will be among the first proteins translated upon water uptake, they likely impact germination potential. Transcriptome changes associated with the increased seed dormancy of the GA-insensitive sly1-2 mutant, and with dormancy loss through sly1-2 after-ripening or constitutive overexpression of the GA receptor (GID1b) were characterized in dry seeds. This experiment used the same seed batches as a previous experiment (E-MTAB-4782) to characterize transcriptional changes associated with the increased seed dormancy and dormancy loss in imbibing seeds. The SLY1 gene encodes the F-box subunit of an SCF E3 ubiquitin ligase needed for GA-triggered proteolysis of DELLA repressors of seed germination. In the sly1-2 mutant, GA-directed DELLA proteolysis cannot occur leading to DELLA protein accumulation and increased dormancy. sly1-2 mutant seeds are fully dormant at 2 weeks of dry storage (0% germination), but germinate well with very long after-ripening (51% germination after 19 months). sly1-2 seed germination can also be rescued by overexpression of the GA receptor, GA-INSENSITIVE DWARF1b (GID1b-OE), which resulted in 74% germination at 2 weeks of dry storage. In this experiment, we sampled dry seeds of wild-type Ler at 2 weeks of dry storage (non-dormant), dormant sly1-2 (2 weeks of dry storage; sly1-2(D)), long after-ripened sly1-2 (non-dormant, 19 months of dry storage; sly1-2(AR)), and sly1-2 GID1b-OE (non-dormant, 2 weeks of dry storage). This experimental design allowed comparison between these transcriptomes in dry seeds to determine if dry seed stored mRNA differences contribute to the dormancy phenotypes observed once seeds are imbibed. Seeds for Ler wt, sly1-2(D), and sly1-2 GID1b-OE were grown alongside each other under the same conditions and after-ripened for 2 weeks. Seeds from sly1-2(AR) were grown under the same conditions in advance of the other lines to allow for the long after-ripening requirement. RNA was extracted using a phenol-chloroform-based extraction from three biological replicates per treatment.

Dataset Information

Total proteome and phosphoproteome of NIL-DOG1 and dog1-1 dry and 6 hours imbibed seed

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