Dataset Information

Total proteome Dry seed Col-0, dwa1 dwa2, YFP:AFP2 (+/-) and YFP:AFP2 in dwa1 dwa2 Background

ABSTRACT: we performed a label-free quantitative mass spectrometry-based proteomic analysis comparing the dry seed proteome of four genotypes in three independent biological replicates: Col-0, dwa1 dwa2 double mutant, and YFP-AFP2 over expressing lines in both Col-0 and dwa1 dwa2 backgrounds. This assay aimed to identified protein accumulation pattern correlating with seed longevity and or ABA resistance during germination.

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Guillaume Née

PROVIDER: PXD030589 | JPOST Repository | Wed Dec 21 00:00:00 GMT 2022

REPOSITORIES: jPOST

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	191017_C19_034_QHF1_Standard_Rev02_%2319_045_GNE106.raw	Raw
	191017_C19_034_QHF1_Standard_Rev02_%2319_045_GNE107.raw	Raw
	191017_C19_034_QHF1_Standard_Rev02_%2319_045_GNE108.raw	Raw
	191017_C19_034_QHF1_Standard_Rev02_%2319_045_GNE109.raw	Raw
	191017_C19_034_QHF1_Standard_Rev02_%2319_045_GNE110.raw	Raw

Items per page:

1 - 5 of 20

Publications

ABI5 binding protein2 inhibits ABA responses during germination without ABA-INSENSITIVE5 degradation.

Lynch Tim T Née Guillaume G Chu Avan A Krüger Thorben T Finkemeier Iris I Finkelstein Ruth R RR

Plant physiology 20220601 2

Overexpression of ABA-INSENSITIVE5 binding proteins (AFPs) results in extreme ABA resistance of seeds and failure to acquire desiccation tolerance, at least in part through effects on chromatin modification. We tested the hypothesis that AFPs promote germination in Arabidopsis (Arabidopsis thaliana) by also functioning as adapters for E3 ligases that ubiquitinate ABI5, leading to its degradation. Interactions between AFPs and two well-characterized classes of E3 ligases targeting ABI5, DWD HYPER ...[more]

PMID: 35258597

Similar Datasets

Project description:Abscisic acid (ABA) is a key hormone for plant growth, development and stress adaptation. Perception of ABA through four types of receptors has been reported. We show here that impairment of ABA perception through the PYR/PYL/RCAR branch reduces vegetative growth and seed production, and leads to a severely open stomata and dramatic ABA insensitive phenotype, even though other branches for ABA perception remain functional. Arabidopsis sextuple mutant impaired in 6 PYR/PYL receptors, namely PYR1, PYL1, PYL2, PYL4, PYL5 and PYL8, was able to germinate and grow even on 100 mM ABA. Whole-rosette stomatal conductance (Gst) measurements revealed that leaf transpiration in the sextuple pyr/pyl mutant was higher than in the ABA-deficient aba3-1 or ABA-insensitive snrk2.6 mutants. The gradually increasing Gst values of plants lacking three, four, five and six PYR/PYLs indicate quantitative regulation of stomatal aperture by this family of receptors. The sextuple mutant lacked ABA-mediated activation of SnRK2s and ABA-responsive gene expression was dramatically impaired as was reported in snrk2.2/2.3/2.6. In summary, these results show that ABA perception by PYR/PYLs plays a major role to regulate seed germination and establishment, basal ABA signaling required for vegetative and reproductive growth, stomatal aperture and transcriptional response to the hormone. Four biological replicates were generated for the 3 genotypes, pyr1pyl1pyl2pyl4pyl5pyl8 sextuple mutant, snrk2.2/2.3/2.6 triple mutant, and Col-0. All the samples were treated with ABA for 3 hours before harvesting. The four Col-0 samples were mix to create an unique reference Col-0 sample. Two comparasion were made, fisrt one, PYR/PYL sextuple mutant versus Col-0 reference sample and second one, SnRK2 triple mutant versus Col-0 reference sample. In each comparasion four biological replicates were made. Replicas number 1 and 2 were labeled with Cy5 for the mutant sample and Cy3 for the Col-0 reference sample, while the other two replicas,#3 and #4, were reversed-labeled.