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Discovering the anticancer mechanism of 52-deoxy-52- methylthioadenosine in cholangiocarcinoma cells using quantitative proteomics analysis

ABSTRACT: Cholangiocarcinoma (CCA) is a highly aggressive cancer originating from the bile duct epithelium. It has been on the rise globally, especially in Asian countries where the liver ûuke infection is prevalent. Current chemotherapy treatments for CCA are often ineûective due to drug resistance. Thus, there is a pressing need for new, eûective anticancer agents. This study explores the potential of 5'-deoxy-5'-methylthioadenosine (MTA), a naturally occurring nucleoside, in treating CCA. MTA has shown promise in inhibiting cell proliferation and invasion in various cancer types, but its eûects on CCA have not been studied.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Sumalee Obchoei

PROVIDER: PXD049118 | JPOST Repository | Sun Feb 02 00:00:00 GMT 2025

REPOSITORIES: jPOST

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			Action	DRS
	KKU-213FR-1_1.mzXML	Mzxml
	KKU-213FR-1_2.mzXML	Mzxml
	KKU-213FR-1_3.mzXML	Mzxml
	KKU-213FR-2_1.mzXML	Mzxml
	KKU-213FR-2_2.mzXML	Mzxml

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Discovering the anticancer mechanism of 52-deoxy-52- methylthioadenosine in cholangiocarcinoma cells using quantitative proteomics analysis

Project description:Cholangiocarcinoma (CCA) is a highly aggressive cancer originating from the bile duct epithelium. It has been on the rise globally, especially in Asian countries where the liver ûuke infection is prevalent. Current chemotherapy treatments for CCA are often ineûective due to drug resistance. Thus, there is a pressing need for new, eûective anticancer agents. This study explores the potential of 5'-deoxy-5'-methylthioadenosine (MTA), a naturally occurring nucleoside, in treating CCA. MTA has shown promise in inhibiting cell proliferation and invasion in various cancer types, but its eûects on CCA have not been studied.

2024-05-15 | PXD049119 | JPOST Repository

Proteomics Analysis of 5-Fluorouracil- and Gemcitabine-Resistant Cholangiocarcinoma Cell Lines: Identification of Potential Therapeutic Targets and Prognostic Markers

Project description:Cholangiocarcinoma (CCA) poses a significant health challenge, especially in Asian countries. Late-stage diagnoses limit the availability of curative surgical options, underscoring the crucial role of chemotherapy. However, drug resistance presents a formidable obstacle, resulting in recurrent tumors. Despite the revolutionary impact of proteomics on cancer research, the exploration of drug-resistant mechanisms in CCA remains unexplored. In this study, we established 5-fluorouracil (5-FU)- and gemcitabine-resistant CCA cell lines, KKU-213FR and KKU-213GR, derived from the KKU-213A parental line. These cell lines exhibited robust drug resistance, heightened migration, and invasion capabilities.

2024-07-03 | PXD049309 | JPOST Repository

Mixed hepatocellular – cholangiocarcinoma derives from liver progenitor cells and depends on senescence and IL6 trans-signaling, single-cell RNA sequencing

Project description:Mixed hepatocellular – cholangiocarcinoma (HCC-CCA) is a highly malignant primary liver cancer of increasing incidence for which the cell of origin and molecular pathogenesis have not yet been fully elucidated. Here, by linking YFP to the progenitor cell specific promoter of Foxl1 in Mdr2-KO mice, we generated a lineage tracing mouse model for liver cancer to monitor the development of mixed HCC-CCA tumors following a prolonged period of liver inflammation. In this model of chronic hepatitis, we demonstrate that liver progenitor cells are the source of mixed HCC-CCA tumors but not of hepatocellular carcinoma (HCC). Moreover, we show that IL6, originating from senescent hepatic cells, is involved in progenitor cell proliferation and development of mixed HCC-CCA tumors via IL6 trans-signaling. These findings could form the basis for the development of novel therapeutic approaches for treatment of mixed HCC-CCA liver cancer.

2023-11-15 | GSE202089 | GEO

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AMPKα Modulation in Cancer Progression: Multilayer Integrative Analysis of the Whole Transcriptome in Asian Gastric Cancer

2012-04-03 | GSE36968 | GEO

AMPKM-NM-1 Modulation in Cancer Progression: Multilayer Integrative Analysis of the Whole Transcriptome in Asian Gastric Cancer

Project description:Gastric cancer is the most common cancer in Asia and most developing countries. To identify the molecular underpinnings of gastric cancer in the Asian population, we applied an RNA-sequencing approach to gastric tumor and noncancerous specimens to quantitatively characterize the entire transcriptome of gastric cancer (including mRNAs and microRNAs). A multi-layer analysis was then developed to identify multiple types of transcriptional aberrations associated with different stages of gastric cancer, including differentially expressed mRNAs, recurrent somatic mutations and key differentially expressed microRNAs. Through this approach, we identified the central metabolic regulator AMPK-M-NM-1 as a potential functional target in Asian gastric cancer. Further, we experimentally demonstrated the translational relevance of this gene as a potential therapeutic target for early-stage gastric cancer in Asian patients. Together, our findings not only provide a valuable information resource for identifying and elucidating the molecular mechanisms of Asian gastric cancer, but also represent a general integrative framework to develop more effective therapeutic targets. Using Life Technologies SOLiDM-bM-^DM-" sequencing platform, we performed transcriptome-wide profiling of gastric cancer samples from 30 anonymous, unrelated Asians of both sexes. Included were six noncancerous gastric tissue samples and 24 gastric tumor samples that represented stages I through IV of tumor development. From the WT-seq protocol we generated a WT-seq dataset of 2.1 billion 50-nt short reads from the 30 samples; Applying the second small RNA-seq protocol to 19 gastric tumor samples (5 of the original 24 yielded insufficient sample amounts) and 6 noncancerous gastric tissue samples resulted in a small RNA-seq dataset.

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Mkango2019 - Dynamics of Breast Cancer under DifferentRates of Chemoradiotherapy

Project description:Dynamics of Breast Cancer under Different Rates of Chemoradiotherapy. Mkango SB1, Shaban N1, Mureithi E1, Ngoma T2. Author information 1 Department of Mathematics, University of Dar es Salaam, P.O. Box 35062, Dar es Salaam, Tanzania. 2 Department of Clinical Oncology, Muhimbili University of Health and Allied Sciences, P.O. Box 65000, Dar es Salaam, Tanzania. Abstract A type of cancer which originates from the breast tissue is referred to as breast cancer. Globally, it is the most common cause of death in women. Treatments such as radiotherapy, chemotherapy, hormone therapy, immunotherapy, and gene therapy are the main strategies in the fight against breast cancer. The present study aims at investigating the effects of the combined radiotherapy and chemotherapy as a way to treat breast cancer, and different treatment approaches are incorporated into the model. Also, the model is fitted to data on patients with breast cancer in Tanzania. We determine new treatment strategies, and finally, we show that when sufficient amount of chemotherapy and radiotherapy with a low decay rate is used, the drug will be significantly more effective in combating the disease while health cells remain above the threshold. Copyright © 2019 Sara B. Mkango et al.

2020-01-20 | MODEL1912120005 | BioModels

Label-free quantitative proteomic approach to investigate the effect of heat stress on Panax ginseng leaves

Project description:Ginseng is one of the well-known medicinal plants, exhibiting diverse medicinal effects. Its roots possess anticancer and anti-aging properties and are being used in the medical systems of East Asian countries. Ginseng is grown in low-light and low-temperature conditions and its growth is strongly inhibited at temperatures above 25 ℃. However, the molecular responses of ginseng to heat stress are currently poorly understood, especially at protein level. Therefore, here we utilized a shotgun proteomics approach to investigate the effect of heat stress on ginseng leaves. Total proteins were isolated from control (25 ℃) and ginseng plants exposed to 35 ℃ for 1 and 3 days and subjected to in-solution trypsin digestion. A total of 3,359 ginseng proteins were identified when searched in an in-house developed RNA-seq (PAC-BIO) database.

2018-12-11 | PXD009124 | Pride

Identification and characterization analysis of the Oriental River Prawn (Macrobrachium nipponense) microRNA response to hypoxia using a deep sequencing approach [miRNA-Seq]

Project description:MicroRNAs (miRNAs) function as regulators in a broad range of phenotypes. The Oriental River Prawn (Macrobrachium nipponense) is an important commercial species that is widely distributed in freshwater and low-salinity estuarine regions of China and other Asian countries. To date, there are no reports describing M. nipponense miRNAs.

2016-06-01 | GSE72417 | GEO

Identification and characterization analysis of the Oriental River Prawn (Macrobrachium nipponense) microRNA response to hypoxia using a deep sequencing approach [miRNA microarray analysis]

2016-06-01 | GSE72416 | GEO