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Identification of the proteins involved in the uptake of coenzyme Q

ABSTRACT: Ubiquinone (UQ), also known as “coenzyme Q” is an essential player in the mitochondrial respiratory electron transport chain as well as a lipid-soluble antioxidant in eukaryotic cells. In this study, we attempted to identify the proteins involved in the pathway by which the exogenous UQ is taken up and accumulated in mitochondria through chemical biology approaches. This project contains MS and MS/MS analysis of the proteins which were specifically labeled by the photo-reactive ubiquinone analogue.

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Masatoshi Murai

PROVIDER: PXD049194 | JPOST Repository | Tue Jun 25 00:00:00 BST 2024

REPOSITORIES: jPOST

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Publications

Identification of proteins involved in intracellular ubiquinone trafficking in Saccharomyces cerevisiae using artificial ubiquinone probe.

Mizutani Mirai M Kuroda Seina S Oku Masahide M Aoki Wataru W Masuya Takahiro T Miyoshi Hideto H Murai Masatoshi M

Biochimica et biophysica acta. Bioenergetics 20240619 4

Ubiquinone (UQ) is an essential player in the respiratory electron transfer system. In Saccharomyces cerevisiae strains lacking the ability to synthesize UQ<sub>6</sub>, exogenously supplied UQs can be taken up and delivered to mitochondria through an unknown mechanism, restoring the growth of UQ<sub>6</sub>-deficient yeast in non-fermentable medium. Since elucidating the mechanism responsible may markedly contribute to therapeutic strategies for patients with UQ deficiency, many attempts have b ...[more]

PMID: 38906315

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