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K562 cell lysates prefractionated by various methods

ABSTRACT: K562 cell lysates were prefractionated by protein reversed-phase chromatography (Prot-RP), SDS-PAGE, and high-pH peptide RP (Pept-RP) to compare their method characteristics. The starting material was prefractionated to 10 fractions for each method. Each prefractionation method was conducted in triplicate to generate 30 raw data per method.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yasushi Ishihama

PROVIDER: PXD049312 | JPOST Repository | Tue Oct 15 00:00:00 BST 2024

REPOSITORIES: jPOST

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Publications

Revisiting Protein Reversed-Phase Chromatography for Bottom-Up Proteomics.

Takagi Shunsuke S Suzuki Nobuyuki N Ishihama Yasushi Y

Journal of proteome research 20240918 10

We revisited protein reversed-phase chromatography (RP), using state-of-the-art RP columns developed for biopharmaceuticals, such as monoclonal antibodies, in order to evaluate the suitability of this methodology as a prefractionation step for bottom-up proteomics. The protein RP prefractionation (Prot-RP) method was compared with two other widely used prefractionation methods, SDS-PAGE and high-pH peptide RP (Pept-RP) by using cell lysates as samples. The overlap between fractions of Prot-RP wa ...[more]

PMID: 39293027

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