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Expression data from Ribonuclease Inhibitor (RI/Rnh1) deficient Human K562 cells

ABSTRACT: Summary: Ribonuclease Inhibitor (RI also known as Rnh1) is a 50 kDa, ubiquitously expressed leucine-rich repeat (LRR) protein. It is localized in cytosol and binds to pancreatic-type ribonucleases and inhibit their function. However, the entire biological role for Rnh1 is unknown. We generated RNH1 knock out K562 cells by CRISPR/Cas9 method. Here we studied differential gene expression from wild type and RNH1 knock out K562 cells by RNA-Seq analysis. Overall design: Total RNA was isolated from wild type and RNH1 deficient K562 cells.

INSTRUMENT(S): Illumina HiSeq 3000

ORGANISM(S): Homo sapiens

SUBMITTER: Cedric Simillion

PROVIDER: E-MTAB-5162 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Ribonuclease inhibitor 1 regulates erythropoiesis by controlling GATA1 translation.

Chennupati Vijaykumar V Veiga Diogo Ft DF Maslowski Kendle M KM Andina Nicola N Tardivel Aubry A Yu Eric Chi-Wang EC Stilinovic Martina M Simillion Cedric C Duchosal Michel A MA Quadroni Manfredo M Roberts Irene I Sankaran Vijay G VG MacDonald H Robson HR Fasel Nicolas N Angelillo-Scherrer Anne A Schneider Pascal P Hoang Trang T Allam Ramanjaneyulu R

The Journal of clinical investigation 20180319 4

Ribosomal proteins (RP) regulate specific gene expression by selectively translating subsets of mRNAs. Indeed, in Diamond-Blackfan anemia and 5q- syndrome, mutations in RP genes lead to a specific defect in erythroid gene translation and cause anemia. Little is known about the molecular mechanisms of selective mRNA translation and involvement of ribosomal-associated factors in this process. Ribonuclease inhibitor 1 (RNH1) is a ubiquitously expressed protein that binds to and inhibits pancreatic- ...[more]

PMID: 29408805

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