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Nuclear and total proteins in THP-1 cell differentiation

ABSTRACT: THP-1 monocyte-like suspension cells can be differentiated into macrophage-like adherent cells by TPA (PMA) treatment. Proteomic analysis was carried out on (i) normal THP-1 cells, (ii) THP-1 cells in which KPNA1 (importin-alpha5) was knocked down or not by siRNA, and (iii) THP-1 TetOn-KPNA2 (importin-alpha1) cells induced or not by Dox. Total and nuclear proteins were prepared from both the monocyte-like and macrophage-like cells and quantified by a label-free method.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Makoto Kimura

PROVIDER: PXD049401 | JPOST Repository | Sat Feb 15 00:00:00 GMT 2025

REPOSITORIES: jPOST

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			Action	DRS
	220615_QE_082_Kimura_01_THP1_susp_total.raw	Raw
	220615_QE_082_Kimura_02_THP1_susp_nuc.raw	Raw
	220615_QE_082_Kimura_03_THP1_MP_NC_total.raw	Raw
	220615_QE_082_Kimura_04_THP1_MP_NC_nuc.raw	Raw
	221013_QE_199_Kimura_01_susp_total_SCX_2nd.raw	Raw

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Publications

Changes in importin levels promote nuclear proteasomal degradation of cell cycle-related proteins during THP-1 monocyte-to-macrophage differentiation.

Kimura Makoto M Ogawa Yutaka Y Motohashi Shoko S Imamoto Naoko N

FEBS letters 20250305 6

Importin family nucleocytoplasmic transport receptors share thousands of cargo proteins. To elucidate cell regulatory mechanisms via transport regulation, we analyzed the levels of transport receptors by western blotting and quantified the total cellular and nuclear proteins during monocyte-to-macrophage differentiation of THP-1 cells using mass spectrometry. Importin-α1 decreased and importin-α5 increased during the differentiation. Cell cycle-related proteins decreased in both whole cells and ...[more]

PMID: 40040501

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