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Stress-induced condensation of TDP-43 in the nucleus

ABSTRACT: TDP-43 is an RNA-binding protein ubiquitously dysregulated in neurodegeneration. We found that under stress conditions, TDP-43 undergoes reversible condensation in the nucleus of cultured cells including human neurons. These condensates (“TCs”) are non-liquid assemblies with low dynamic properties. Their proteomic analysis and subsequent comparison with the normal (soluble) TDP-43 interactome revealed that upon condensate partitioning, TDP-43 dissociates from its normal protein binding partners. We propose that the main function of TCs is rapid but reversible inactivation of TDP-43 during stress. This can help fine-tune the splicing patterns under stress to enable efficient stress response and survival.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Tatyana Shelkovnikova

PROVIDER: PXD052963 | JPOST Repository | Mon Jun 10 00:00:00 BST 2024

REPOSITORIES: jPOST

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			Action	DRS
	GFP_1.raw	Raw
	GFP_2.raw	Raw
	Rachel%20Hodgson%20110823_GFP1.xlsx	Xlsx
	Rachel%20Hodgson%20110823_GFP2.xlsx	Xlsx
	Rachel%20Hodgson%20110823_TC2-1.xlsx	Xlsx

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