Proteomics

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Proteomics analysis of proteins co-immunoprecipitated with MIDN-Flag in PC-12 cells

ABSTRACT: We have previously identified midnolin (MIDN) as a genetic risk factor for Parkinson's disease. However, the molecular entity of MIDN that regulates neuronal phenotypes remains unclear. To investigate the molecular function of MIDN, we examined proteins associated with MIDN in PC-12 cells. We performed LC-MS/MS analysis of flag-immunoprecipitated samples from the nuclear fraction of PC-12 cells expressing flag-tagged MIDN (MIDN-Flag) or pcDNA3.1(+) (the empty vector) treated with MG132 (5 μM, 6 h) and nerve growth factor (500 ng/mL, 2 h).

ORGANISM(S): Rattus Rattus (black Rat)

SUBMITTER: Yutaro Obara 

PROVIDER: PXD053265 | JPOST Repository | Fri Sep 13 00:00:00 BST 2024

REPOSITORIES: jPOST

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20240610_QE2_HTSPAL_ESI12cmRT_sand_AcOH_PE_2024_088_Alignment.mzTab Mztab
20240610_QE2_HTSPAL_ESI12cmRT_sand_AcOH_PE_2024_088_e1.raw Raw
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Publications

Midnolin, a Genetic Risk Factor for Parkinson's Disease, Promotes Neurite Outgrowth Accompanied by Early Growth Response 1 Activation in PC12 Cells.

Chiba Ayano A   Kato Chisato C   Nakagawa Tadashi T   Osaki Tsukasa T   Nakamura Kohei K   Norota Ikuo I   Nagashima Mikako M   Hosoi Toru T   Ishii Kuniaki K   Obara Yutaro Y  

Molecular and cellular biology 20240912 11

Parkinson's disease (PD) is an age-related progressive neurodegenerative disease. Previously, we identified midnolin (<i>MIDN</i>) as a genetic risk factor for PD. Although <i>MIDN</i> copy number loss increases the risk of PD, the molecular function of MIDN remains unclear. To investigate the role of MIDN in PD, we established monoclonal <i>Midn</i> knockout (KO) PC12 cell models. <i>Midn</i> KO inhibited neurite outgrowth and neurofilament light chain (<i>Nefl</i>) gene expression. Although MI  ...[more]

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