Proteomics

Dataset Information

0

Proteomics analysis of proteins co-immunoprecipitated with MIDN-Flag in PC-12 cells

ABSTRACT: We have previously identified midnolin (MIDN) as a genetic risk factor for Parkinson's disease. However, the molecular entity of MIDN that regulates neuronal phenotypes remains unclear. To investigate the molecular function of MIDN, we examined proteins associated with MIDN in PC-12 cells. We performed LC-MS/MS analysis of flag-immunoprecipitated samples from the nuclear fraction of PC-12 cells expressing flag-tagged MIDN (MIDN-Flag) or pcDNA3.1(+) (the empty vector) treated with MG132 (5 μM, 6 h) and nerve growth factor (500 ng/mL, 2 h).

ORGANISM(S): Rattus Rattus (black Rat)

SUBMITTER: Yutaro Obara 

PROVIDER: PXD053265 | JPOST Repository | Fri Sep 13 00:00:00 BST 2024

REPOSITORIES: jPOST

Json Xml
altmetric image

Publications

Midnolin, a Genetic Risk Factor for Parkinson's Disease, Promotes Neurite Outgrowth Accompanied by Early Growth Response 1 Activation in PC12 Cells.

Chiba Ayano A   Kato Chisato C   Nakagawa Tadashi T   Osaki Tsukasa T   Nakamura Kohei K   Norota Ikuo I   Nagashima Mikako M   Hosoi Toru T   Ishii Kuniaki K   Obara Yutaro Y  

Molecular and cellular biology 20240912

Parkinson's disease (PD) is an age-related progressive neurodegenerative disease. Previously, we identified midnolin (<i>MIDN</i>) as a genetic risk factor for PD. Although <i>MIDN</i> copy number loss increases the risk of PD, the molecular function of MIDN remains unclear. To investigate the role of MIDN in PD, we established monoclonal <i>Midn</i> knockout (KO) PC12 cell models. <i>Midn</i> KO inhibited neurite outgrowth and neurofilament light chain (<i>Nefl</i>) gene expression. Although MI  ...[more]

Similar Datasets

Transcriptomics Gene expression profiling of FaDu cells overexpressing TINCR-HA-FLAG
2024-07-16 | GSE180301 | GEO
Transcriptomics Transcription profiling of human H1299 cells and Saos-2 cells (p53 null cells) transfected with Flag vector, Flag-p53 WT or Flag-p53 SA
2010-10-14 | E-MEXP-2556 | biostudies-arrayexpress
Transcriptomics Identify Flag-bound transcripts via RIP-seq
2021-10-28 | GSE182620 | GEO
Proteomics RNF5 interactome
2018-11-25 | MSV000083160 | MassIVE
Genomics FLAG-tagged FOXM1A Plasmid
| PRJEB24606 | ENA
Genomics Genome-wide maps of Tet1 binding in [WT, Tet1-Flag] and [Dnmt3a-/-, Tet1-Flag] J1 ES cells
2018-05-16 | GSE100955 | GEO
Genomics ChIP-seq of PtrVCS2 fuse FLAG overexpression plants
2022-11-11 | GSE201005 | GEO
Transcriptomics Expression data wheat Flag Leaf at anthesis
2013-07-01 | GSE42000 | GEO
Genomics ChIP-seq of FLAG-tagged SMARCAD1 in female mouse embryonic stem cells (PGK12.1 XX)
2019-02-17 | E-MTAB-7014 | biostudies-arrayexpress
Proteomics,Multiomics Integrative Genomics Positions MKRN1 as a Novel Ribonucleoprotein within the Embryonic Stem Cell Gene Regulatory Network
2018-10-19 | PXD001191 | Pride