Dataset Information

Allergen marker discovery and quantification

ABSTRACT: We developed a high-resolution accurate mass (HRAM)-LC-MS/MS method using the same food extract used in the ELISA method and an MS sample preparation kit. Multiple food allergen analysis was performed using 1, 5, 10, and 20 ppm of allergen-incurred processed foods. Overall, a strong correlation was observed between the measured values of HRAM-LC-MS/MS and ELISA, demonstrating the applicability of multi-allergen analysis using LC-MS.

ORGANISM(S): Cellular Organisms

SUBMITTER: Yuriko Oyama

PROVIDER: PXD053465 | JPOST Repository | Wed Jul 31 00:00:00 BST 2024

REPOSITORIES: jPOST

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Publications

Improved multi-food allergen analysis of processed foods using HRAM-LC-MS/MS with an ELISA-validated extraction solution and MS sample prep kit.

Oyama Yuriko Y Hamasaka Tomoko T Okada Hideki H Nagashima Yoshiki Y Morita Minoru M

Analytical and bioanalytical chemistry 20240730 23

Food allergens in processed foods are affected by heating, processing, and the food matrix. To conduct highly reliable tests, extracting allergens into test solutions is necessary for appropriate detection. In addition to the commonly used enzyme-linked immunosorbent assay (ELISA), liquid chromatography-mass spectrometry (LC-MS), which has the advantage of simultaneously detecting multiple allergens in foods, is being increasingly used. When managing food allergens at food manufacturing sites, o ...[more]

PMID: 39078454

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			Action	DRS
	20220512_SwissProt.fasta	Fasta
	220516_Buckwheat.pdResult	Other
	220516_Egg.pdResult	Other
	220516_Milk.pdResult	Other
	220516_Peanuts.pdResult	Other