Dataset Information

Protein expression in heated rat lens using shotgun proteomics

ABSTRACT: Previous studies have reported that a strong correlation between the estimated cumulative thermal exposure in the crystalline lens and the incidence of nuclear cataracts; however, the precise relationship between temperature and cataracts remains to be fully elucidated. In the present study, the shotgun liquid chromatography/mass spectroscopy-based global proteomic approach was applied to investigate cataract-inducing factors in lens cultured at normal (35.0˚C) and slightly warmer (37.5˚C) conditions. In the rat lens, 190 proteins (total) were identified. Of these, 48 proteins (25.3%) were found in lenses cultured at both 35.0˚C and 37.5˚C. Moreover, 85 proteins (44.7%) were unique to lenses cultured at 35.0˚C, while 57 proteins (30.0%) were unique to lenses cultured at 37.5˚C. Protein expression changes in rat lenses cultured at 37.5˚C were examined using a label-free semiquantitative approach that uses spectral counting and Gene Ontology analysis. Filensin and vimentin protein expression, key factors in maintaining lens structure, were decreased. These findings may serve as a valuable indicator for elucidating the relationship between temperature and the onset of nuclear cataracts.

ORGANISM(S): Rattus

SUBMITTER: Tetsushi Yamamoto

PROVIDER: PXD056067 | JPOST Repository | Sun Sep 22 00:00:00 BST 2024

REPOSITORIES: jPOST

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Semiquantitative analysis of protein expression in heated rat lens using shotgun proteomics.

Otake Hiroko H Masuda Shuya S Yamamoto Tetsushi T Miyata Yoshiki Y Nakazawa Yosuke Y Yamamoto Naoki N Taga Atsushi A Sasaki Hiroshi H Nagai Noriaki N

Molecular medicine reports 20241114 1

Previous studies have reported that a strong correlation between the estimated cumulative thermal exposure in the crystalline lens and the incidence of nuclear cataracts; however, the precise relationship between temperature and cataracts remains to be fully elucidated. In the present study, the shotgun liquid chromatography/mass spectroscopy‑based global proteomic approach was applied to investigate cataract‑inducing factors in lens cultured at normal (35.0˚C) and slightly warmer (37.5˚C) condi ...[more]

PMID: 39540352

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