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Peptide mapping and intact mass analysis of RBD protein from a SARS-CoV-2 omicron variant (RBDom)

ABSTRACT: RBD protein from the SARS-CoV-2 omicron variant (RBDom) was produced in Komagataella phaffii. Peptide mapping and intact mass analysis of RBDom were performed by LC/MS. Proteins were identified using BioPharma Finder software.

ORGANISM(S): Komagataella Phaffii

SUBMITTER: Noritaka Hashii

PROVIDER: PXD057342 | JPOST Repository | Wed Oct 30 00:00:00 GMT 2024

REPOSITORIES: jPOST

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			Action	DRS
	20230925_01063809316843_Intact_kobe_SARS_CoV2_RBD_omicron_PNGaseF_Rapid_OverWeek_SF30_0590B_5uginj.raw	Raw
	20230925_01063809316843_Intact_kobe_SARS_CoV2_RBD_omicron_PNGaseF_Rapid_OverWeek_SF30_0590B_5uginj_Intact%20mass%20analysis.pdf	Pdf
	20230927_SN01703111815178_CSH_C18_HCD_kobe_SARS_CoV2_RBD_omicron_MAM_PNGaseFON_ChymoON_HLB_3uginj_Lock_MS_off.raw	Raw
	20230927_SN01703111815178_CSH_C18_HCD_kobe_SARS_CoV2_RBD_omicron_MAM_PNGaseFON_ChymoON_HLB_3uginj_Lock_MS_off_peptide_mapping.pmf	Other

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Publications

Designing strong inducible synthetic promoters in yeasts.

Tominaga Masahiro M Shima Yoko Y Nozaki Kenta K Ito Yoichiro Y Someda Masataka M Shoya Yuji Y Hashii Noritaka N Obata Chihiro C Matsumoto-Kitano Miho M Suematsu Kohei K Matsukawa Tadashi T Hosoya Keita K Hashiba Noriko N Kondo Akihiko A Ishii Jun J

Nature communications 20241219 1

Inducible promoters are essential for precise control of target gene expression in synthetic biological systems. However, engineering eukaryotic promoters is often more challenging than engineering prokaryotic promoters due to their greater mechanistic complexity. In this study, we describe a simple and reliable approach for constructing strongly inducible synthetic promoters with minimum leakiness in yeasts. The results indicate that the leakiness of yeast-inducible synthetic promoters is prima ...[more]

PMID: 39702268

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