Dataset Information

Alkylation Damage by Lipid Electrophiles-- HNE-induced expression in RKO cells

ABSTRACT: 4-hydroxynonenal (HNE) (UniMod 53) (+156.115030 Da on Cys, His, Lys) reduced 4-Hydroxynonenal (UniMod 335(+158.130680 Da on Cys, His, Lys) These data were additions to the set for the paper Alkylation Damage by Lipid Electrophiles Targets Functional Protein Systems by Codreanu et al, Mol. Cell. Proteomics (2013 or 2014).

INSTRUMENT(S): LTQ

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Daniel C. Liebler

PROVIDER: MSV000078512 | MassIVE | Wed Dec 18 08:06:00 GMT 2013

REPOSITORIES: MassIVE

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Alkylation damage by lipid electrophiles targets functional protein systems.

Codreanu Simona G SG Ullery Jody C JC Zhu Jing J Tallman Keri A KA Beavers William N WN Porter Ned A NA Marnett Lawrence J LJ Zhang Bing B Liebler Daniel C DC

Molecular & cellular proteomics : MCP 20140115 3

Protein alkylation by reactive electrophiles contributes to chemical toxicities and oxidative stress, but the functional impact of alkylation damage across proteomes is poorly understood. We used Click chemistry and shotgun proteomics to profile the accumulation of proteome damage in human cells treated with lipid electrophile probes. Protein target profiles revealed three damage susceptibility classes, as well as proteins that were highly resistant to alkylation. Damage occurred selectively acr ...[more]

PMID: 24429493

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