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AP-MS MudPIT run of Ino80 complex

ABSTRACT: File name explanation: Sc_Ino80_TAP_V2_P1_Ti_101: Sc = Saccharomyces cerevisiae (specie) Ino80 = bait protein TAP = tag V2 = lab instrument name(Velos_Elite2) P1 = biological replicate 1 Ti = LysC+Trypsin digestion 101 = MudPIT run 1 from 1st technical replicate

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932)

SUBMITTER: Michael P. Washburn, Craig L. Peterson, Thomas Walz

PROVIDER: MSV000079018 | MassIVE | Wed Jan 28 09:20:00 GMT 2015

SECONDARY ACCESSION(S): PXD001742

REPOSITORIES: MassIVE

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Publications

Structural analyses of the chromatin remodelling enzymes INO80-C and SWR-C.

Watanabe Shinya S Tan Dongyan D Lakshminarasimhan Mahadevan M Washburn Michael P MP Hong Eun-Jin Erica EJ Walz Thomas T Peterson Craig L CL

Nature communications 20150512

INO80-C and SWR-C are conserved members of a subfamily of ATP-dependent chromatin remodelling enzymes that function in transcription and genome-maintenance pathways. A crucial role for these enzymes is to control chromosomal distribution of the H2A.Z histone variant. Here we use electron microscopy (EM) and two-dimensional class averaging to demonstrate that these remodelling enzymes have similar overall architectures. Each enzyme is characterized by a dynamic 'tail' domain and a compact 'head' ...[more]

PMID: 25964121

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