Dataset Information

AP-MS MudPIT run of yeast R2TP complex

ABSTRACT: File name explanation: Sc_Rtt101_TAP_P1_Ti_101: Sc = Saccharomyces cerevisiae (specie) Rtt101 = bait protein TAP = tag L1 = lab instrument name(LTQ1) P1 = biological replicate 1 Ti = LysC+Trypsin digestion 101 = MudPIT run 1 from 1st technical replicate rvb1_his_sf21_E1_P1_Ti_101: Sf21 = Spodoptera frugiperda rvb1 = protein with tag his = 6x HIS tag E1 = elution number 1 L1 = lab instrument name(LTQ1) P1 = biological replicate 1 Ti = LysC+Trypsin digestion 101 = MudPIT run 1 from 1st technical replicate

OTHER RELATED OMICS DATASETS IN: PRJNA284918

INSTRUMENT(S): LTQ, LTQ XL

ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932)

SUBMITTER: Michael P. Washburn

PROVIDER: MSV000079332 | MassIVE |

SECONDARY ACCESSION(S): PXD004823

REPOSITORIES: MassIVE

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Publications

Proteomic and Genomic Analyses of the Rvb1 and Rvb2 Interaction Network upon Deletion of R2TP Complex Components.

Lakshminarasimhan Mahadevan M Boanca Gina G Banks Charles A S CA Hattem Gaye L GL Gabriel Ana E AE Groppe Brad D BD Smoyer Christine C Malanowski Kate E KE Peak Allison A Florens Laurence L Washburn Michael P MP

Molecular & cellular proteomics : MCP 20160201 3

The highly conserved yeast R2TP complex, consisting of Rvb1, Rvb2, Pih1, and Tah1, participates in diverse cellular processes ranging from assembly of protein complexes to apoptosis. Rvb1 and Rvb2 are closely related proteins belonging to the AAA+ superfamily and are essential for cell survival. Although Rvbs have been shown to be associated with various protein complexes including the Ino80 and Swr1chromatin remodeling complexes, we performed a systematic quantitative proteomic analysis of thei ...[more]

PMID: 26831523

Similar Datasets

Project description:Dear Sir or Madam, we report an in-depth proteogenomics study of Helicobacter pylori strain 26695 and provide the supporting MS data via ProteomExchange. The study includes 2 biological replicates with 6 different datasets: G1: in-gel digestion with trypsin, replicate 1 G2: in-gel digestion with trypsin, replicate 2 T1: SEC fractionation of low molecular weight (LMW) proteins and subsequent trypsin digestion, replicate 1 T2: SEC fractionation of LMW proteins and subsequent trypsin digestion, replicate 2 A1: SEC fractionation of LMW proteins and subsequent AspN digestion, replicate 1 A2: SEC fractionation of LMW proteins and subsequent AspN digestion, replicate 2 L1: SEC fractionation of LMW proteins and subsequent LysC digestion, replicate 1 L2: SEC fractionation of LMW proteins and subsequent LysC digestion, replicate 2 In our proteogenomics approach, we could identify four previously missing protein annotations and were able to correct sequences of six protein coding regions. Furthermore we identified signal peptidase cleavage sites for 72 different proteins. MGFs were generated by Maxquant 1.1 [1] using recalibration of peptide parent masses. For PRIDE (http://www.ebi.ac.uk/pride) submission, we made an additional database search with Mascot and X!Tandem using the SearchGUI [2]. Therefore we searched against a NCBI database of H. pylori strain 26695 complemented with the sequence corrections, signal peptide cleavage sites and missing annotations with the same configurations as described in materials and methods. For pride xml export we used the software PeptideShaker (http://code.google.com/p/peptide-shaker/). The complemented database has entries which will be submitted to the UniProtKB via SPIN. The entries have the according SPIN number as accession number. The NCBI accession numbers for the shortened sequences due to signal peptide cleavage are extended with “_1”. The fasta database is added to the submission. For additional information, please contact me: stephan.mueller@ufz.de Yours sincerely, Stephan Mueller References: [1] Cox J, Neuhauser N, Michalski A, Scheltema RA, Olsen JV, Mann M. Andromeda: a peptide search engine integrated into the MaxQuant environment. Journal of proteome research. 2011;10:1794-805. [2] Vaudel M, Barsnes H, Berven FS, Sickmann A, Martens L. SearchGUI: An open-source graphical user interface for simultaneous OMSSA and X!Tandem searches. Proteomics. 2011;11:996-9.