Project description:Several oral bacterial species were grown anaerobically and fed either glucose or lactate in SHI medium (for method see: Edlund et al., 2013, Edlund et al, 2015 - see http://www.microbiomejournal.com/content/pdf/2049-2618-1-25.pdf & http://www.nature.com/ismej/journal/vaop/ncurrent/full/ismej201572a.html). Biofilm samples for LCMS were collected at different time points from growth wells. After growth was established in SHI medium, biofilms were washed with minimal medium and supplemented with either glucose or lactate (Veillonella species) (see Edlund et al., 2013, Edlund et al., 2015). Samples were collected both after the initial biofilm establishment in SHI medium and after the addition of the supplemented cdm medium. According to gnps network analyses and data sorting exercises: 1) ~10% of this data is reproducible; 2) several of the present masses can also be detected both in SHI and cdm medium. mzXML files for media is not uploaded here (i.e. no media subtraction can be employed using these files only).
Project description:The purpose of this study was to isolate NCSCs from oral mucosa using the neurosphere technique. Total RNA from human oral mucosa stromal cells and sphere-formig oral mucosa stromal cells was collected and compared at their gene expression level. Samples from 3 patients were analysed.
Project description:To investigate the virological properties of SARS-CoV-2 variants, we amplified the clinical isolates of an early pandemic D614G-bearing isolate (B.1.1 lineage, strain TKYE610670; GISAID ID: EPI_ISL_479681), a Delta isolate (B.1.617.2 lineage, strain TKYTK1734; GISAID ID: EPI_ISL_2378732) and an Omicron isolate (BA.1 lineage, strain TY38-873; GISAID ID: EPI_ISL_7418017) and prepared the working viruses.
2022-01-07 | GSE192472 | GEO
Project description:Subgingival SHI-medium in vitro Biofilm Sequences