Dataset Information

Human Cardiac Stem Cells Receptome

ABSTRACT: Human cardiac stem cells (hCSC) express a portfolio of plasma membrane receptors that are involved in the regulatory auto/paracrine feedback loop mechanism of activation of these cells, and consequently contribute to myocardial regeneration. In order to attain a comprehensive description of hCSC receptome and overcoming the inability demonstrated by other technologies applied in receptor identification, mainly due to the transmembrane nature, high hydrophobic character and relative low concentration of these proteins, we have exploited and improved a proteomics workflow. This approach was based on the enrichment of hCSC plasma membrane fraction and addition of pre-fractionation steps prior to MS analysis. More than one hundred plasma membrane receptors were identified. The data reported herein constitute a valuable source of information to further understand cardiac stem cells activation mechanisms and the subsequent cardiac repair process.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr Paula M. Alves

PROVIDER: MSV000079549 | MassIVE | Tue Mar 01 13:46:00 GMT 2016

SECONDARY ACCESSION(S): PXD001117

REPOSITORIES: MassIVE

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Publications

Exploring analytical proteomics platforms toward the definition of human cardiac stem cells receptome.

Gomes-Alves Patrícia P Serra Margarida M Brito Catarina C R-Borlado Luis L López Juan A JA Vázquez Jesús J Carrondo Manuel J T MJ Bernad António A Alves Paula M PM

Proteomics 20150205 7

Human cardiac stem cells (hCSC) express a portfolio of plasma membrane receptors that are involved in the regulatory auto/paracrine feedback loop mechanism of activation of these cells, and consequently contribute to myocardial regeneration. In order to attain a comprehensive description of hCSC receptome and overcoming the inability demonstrated by other technologies applied in receptor identification, mainly due to the transmembrane nature, high hydrophobic character and relative low concentra ...[more]

PMID: 25504917

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