Proteomics

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Human Cardiac Stem Cells Receptome

ABSTRACT: Human cardiac stem cells (hCSC) express a portfolio of plasma membrane receptors that are involved in the regulatory auto/paracrine feedback loop mechanism of activation of these cells, and consequently contribute to myocardial regeneration. In order to attain a comprehensive description of hCSC receptome and overcoming the inability demonstrated by other technologies applied in receptor identification, mainly due to the transmembrane nature, high hydrophobic character and relative low concentration of these proteins, we have exploited and improved a proteomics workflow. This approach was based on the enrichment of hCSC plasma membrane fraction and addition of pre-fractionation steps prior to MS analysis. More than one hundred plasma membrane receptors were identified. The data reported herein constitute a valuable source of information to further understand cardiac stem cells activation mechanisms and the subsequent cardiac repair process.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Heart, Stem Cell, Adult Stem Cell

SUBMITTER: Patrícia Gomes-Alves  

LAB HEAD: Paula M. Alves

PROVIDER: PXD001117 | Pride | 2015-01-30

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
JA_Toran_R1g.pep.xml Pepxml
JA_Toran_R1g.prot.xml Xml
JA_Toran_R2g.pep.xml Pepxml
JA_Toran_R2g.prot.xml Xml
JA_Toran_R3g.pep.xml Pepxml
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Publications

Exploring analytical proteomics platforms toward the definition of human cardiac stem cells receptome.

Gomes-Alves Patrícia P   Serra Margarida M   Brito Catarina C   R-Borlado Luis L   López Juan A JA   Vázquez Jesús J   Carrondo Manuel J T MJ   Bernad António A   Alves Paula M PM  

Proteomics 20150205 7

Human cardiac stem cells (hCSC) express a portfolio of plasma membrane receptors that are involved in the regulatory auto/paracrine feedback loop mechanism of activation of these cells, and consequently contribute to myocardial regeneration. In order to attain a comprehensive description of hCSC receptome and overcoming the inability demonstrated by other technologies applied in receptor identification, mainly due to the transmembrane nature, high hydrophobic character and relative low concentra  ...[more]

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