Dataset Information

Analysis of Reproducibility of Proteome Coverage and Quantitation Using Isobaric Mass Tags (iTRAQ and TMT)

ABSTRACT: This study aimed to compare the depth and reproducibility of total proteome and differentially expressed protein coverage in technical duplicates and triplicates using iTRAQ 4-plex, iTRAQ 8-plex and TMT 6-plex reagents.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Phaeosphaeria Nodorum Sn15 (ncbitaxon:321614)

SUBMITTER: Richard J Lipscombe

PROVIDER: MSV000079594 | MassIVE | Thu Mar 17 21:59:00 GMT 2016

SECONDARY ACCESSION(S): PXD003807

REPOSITORIES: MassIVE

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Analysis of Reproducibility of Proteome Coverage and Quantitation Using Isobaric Mass Tags (iTRAQ and TMT).

Casey Tammy M TM Khan Javed M JM Bringans Scott D SD Koudelka Tomas T Takle Pari S PS Downs Rachael A RA Livk Andreja A Syme Robert A RA Tan Kar-Chun KC Lipscombe Richard J RJ

Journal of proteome research 20161207 2

This study aimed to compare the depth and reproducibility of total proteome and differentially expressed protein coverage in technical duplicates and triplicates using iTRAQ 4-plex, iTRAQ 8-plex, and TMT 6-plex reagents. The analysis was undertaken because comprehensive comparisons of isobaric mass tag reproducibility have not been widely reported in the literature. The highest number of proteins was identified with 4-plex, followed by 8-plex and then 6-plex reagents. Quantitative analyses revea ...[more]

PMID: 28152591

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