Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
SUBMITTER: Dr Ulf �rom
PROVIDER: MSV000080265 | MassIVE | Fri Oct 21 13:55:00 BST 2016
SECONDARY ACCESSION(S): PXD003664
REPOSITORIES: MassIVE
Conrad Thomas T Albrecht Anne-Susann AS de Melo Costa Veronica Rodrigues VR Sauer Sascha S Meierhofer David D Ørom Ulf Andersson UA
Nature communications 20160404
Novel RNA-guided cellular functions are paralleled by an increasing number of RNA-binding proteins (RBPs). Here we present 'serial RNA interactome capture' (serIC), a multiple purification procedure of ultraviolet-crosslinked poly(A)-RNA-protein complexes that enables global RBP detection with high specificity. We apply serIC to the nuclei of proliferating K562 cells to obtain the first human nuclear RNA interactome. The domain composition of the 382 identified nuclear RBPs markedly differs from ...[more]