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Properties of the Cuticular Proteins of Anopheles Gambiae as Revealed by Serial Extraction of Adults

ABSTRACT: How cuticular proteins (CPs) interact with chitin and with each other in the cuticle remains unresolved. We employed LC-MS/MS to identify CPs from 5-6 day-old adults of Anopheles gambiae released after serial extraction with PBS, EDTA, 2-8M urea, and SDS as well as those that remained unextracted. Results were compared to published data on time of transcript abundance, localization of proteins within structures and within the cuticle, as well as properties of individual proteins, length, pI, percent histidine, tyrosine, glutamine, and number of AAP[A/V/L] repeats. Thirteen proteins were solubilized completely, all were CPRs. Eleven were identified in both soluble fractions and the final pellet, including 5 from other CP families. Forty-four were only detected from the final pellet. These included CPRs and members of the CPAP1, CPF, CPFL, CPLCA, CPLCG, CPLCP, and TWDL families, as well as several low complexity CPs, not assigned to families. For a given protein, many histidines or tyrosines or glutamines appear to be potential participants in cross-linking since we could not identify consistent absence of individual peptides bearing these residues. We failed to recover peptides from the amino-terminus of any CP. Whether this implicates that location in sclerotization or some modification that prevents detection is not known. Soluble CPRs had lower isoelectric points than those that remained in the final pellet; most members of other CP families had isoelectric points of 8 or higher.

INSTRUMENT(S): Orbitrap Fusion, LTQ Orbitrap Elite

ORGANISM(S): Anopheles Gambiae (ncbitaxon:7165)

SUBMITTER: Dr. Judith H. Willis

PROVIDER: MSV000080580 | MassIVE | Mon Feb 27 14:39:00 GMT 2017

SECONDARY ACCESSION(S): PXD005983

REPOSITORIES: MassIVE

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Project description:The exoskeleton gives to insects its shape, waterproofing and a range of other roles, besides serving as means of locomotion, the motor function being accomplished by the attachment of somatic muscles. Exoskeleton is renewed every time the insect molts under the control of ecdysteroid hormones. The last molt, which in the holometabolous transforms pupae in adults, involves biosynthesis of the definitive exoskeleton by the subjacent epidemis, followed by progressive differentiation. We used whole genome-based oligonucleotide microarray hybridization and RNA extracted from the honey bee thoracic dorsum, at three time-points of the pupal-to-adult molt, as a strategy to screen genes involved in exoskeleton formation. Gene ontology analysis separated the differentially expressed genes (DEGs) in distinct Biological Processes and Molecular Functions dependently on the time-point approached, thus revealing the functional categories required for adult exoskeleton formation. Approximately 546 of a list of 1253 unique DEGs were up-regulated in the thoracic dorsum during adult cuticle formation, including 23 among the 24 identified cuticle protein (CP) genes, and 29 muscle-related genes. Conserved sequence motifs allowed to include the CP genes in the CPR, Tweedle, Apidermin, CPF, CPLCP1 and Analogous-to-Peritrophins families. Two genes, which do not pertain to any known CP families, were abundantly expressed in the epidermis during adult cuticle formation as shown by in situ hybridization, thus strongly suggesting that they are genuine CP genes. The search for cis-regulatory motifs in the 5’untranslated region of the DEGs revealed potential binding sites for known transcript factors. Several of them were shared by CP genes and thoracic muscle genes, thus allowing to infer that they are co-regulated during differentiation of the thoracic exoskeleton. Together, these data add new information on molecular aspects of exoskeleton formation in the context of the ecdysteroid-coordinated pupal-to-adult molt.

Project description:The exoskeleton gives to insects its shape, waterproofing and a range of other roles, besides serving as means of locomotion, the motor function being accomplished by the attachment of somatic muscles. Exoskeleton is renewed every time the insect molts under the control of ecdysteroid hormones. The last molt, which in the holometabolous transforms pupae in adults, involves biosynthesis of the definitive exoskeleton by the subjacent epidemis, followed by progressive differentiation. We used whole genome-based oligonucleotide microarray hybridization and RNA extracted from the honey bee thoracic dorsum, at three time-points of the pupal-to-adult molt, as a strategy to screen genes involved in exoskeleton formation. Gene ontology analysis separated the differentially expressed genes (DEGs) in distinct Biological Processes and Molecular Functions dependently on the time-point approached, thus revealing the functional categories required for adult exoskeleton formation. Approximately 546 of a list of 1253 unique DEGs were up-regulated in the thoracic dorsum during adult cuticle formation, including 23 among the 24 identified cuticle protein (CP) genes, and 29 muscle-related genes. Conserved sequence motifs allowed to include the CP genes in the CPR, Tweedle, Apidermin, CPF, CPLCP1 and Analogous-to-Peritrophins families. Two genes, which do not pertain to any known CP families, were abundantly expressed in the epidermis during adult cuticle formation as shown by in situ hybridization, thus strongly suggesting that they are genuine CP genes. The search for cis-regulatory motifs in the 5M-bM-^@M-^Yuntranslated region of the DEGs revealed potential binding sites for known transcript factors. Several of them were shared by CP genes and thoracic muscle genes, thus allowing to infer that they are co-regulated during differentiation of the thoracic exoskeleton. Together, these data add new information on molecular aspects of exoskeleton formation in the context of the ecdysteroid-coordinated pupal-to-adult molt. The microarray experiments compared differential gene expression in the thoracic integuments of newly-ecdysed pupae (Pw phase), pupae-in-apolysis (Pp phase) and pharate-adults (Pbl phase) collected at the same time from a single honey bee colony. Two separate pools of 10 thoracic integuments each were prepared for each developmental phase (Pw, Pp and Pbl). Dye swaps were done for each comparison and two slides were used to evaluate the differential expression.

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Properties of the Cuticular Proteins of Anopheles Gambiae as Revealed by Serial Extraction of Adults

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