Dataset Information

Inflammatory signaling in human Tuberculosis granulomas is spatially organized

ABSTRACT: Granulomas are the pathological hallmark of tuberculosis (TB). However, their function and mechanisms of formation remain poorly understood. To understand the role of granulomas in TB, we analyzed the proteomes of granulomas from TB patients in an unbiased fashion. Using laser capture microdissection and mass spectrometry , we generated detailed molecular maps of human granulomas. We found that the centers of granulomas possess a pro-inflammatory environment characterized by anti-microbial peptides, ROS and pro-inflammatory eicosanoids. Conversely, the tissue surrounding the caseum possesses a comparatively anti-inflammatory signature.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Matthias Mann

PROVIDER: MSV000080720 | MassIVE | Wed Mar 29 00:52:00 BST 2017

SECONDARY ACCESSION(S): PXD003646

REPOSITORIES: MassIVE

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Publications

Inflammatory signaling in human tuberculosis granulomas is spatially organized.

Marakalala Mohlopheni J MJ Raju Ravikiran M RM Sharma Kirti K Zhang Yanjia J YJ Eugenin Eliseo A EA Prideaux Brendan B Daudelin Isaac B IB Chen Pei-Yu PY Booty Matthew G MG Kim Jin Hee JH Eum Seok Yong SY Via Laura E LE Behar Samuel M SM Barry Clifton E CE Mann Matthias M Dartois Véronique V Rubin Eric J EJ

Nature medicine 20160404 5

Granulomas are the pathological hallmark of tuberculosis (TB). However, their function and mechanisms of formation remain poorly understood. To understand the role of granulomas in TB, we analyzed the proteomes of granulomas from subjects with tuberculosis in an unbiased manner. Using laser-capture microdissection, mass spectrometry and confocal microscopy, we generated detailed molecular maps of human granulomas. We found that the centers of granulomas have a pro-inflammatory environment that i ...[more]

PMID: 27043495

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