Dataset Information

Proteomics of U1810 cells upon treatment with microRNAs with an AAGUGC seed motif.

ABSTRACT: microRNA dysregulation is a common feature of cancer cells, but the complex roles of microRNAs in cancer are not fully elucidated. Here we used functional genomics to identify oncogenic microRNAs in non-small cell lung cancer and to evaluate their impact on response to EGFR targeting therapy. Our data demonstrate that microRNAs with an AAGUGC-motif in their seed-sequence increase both cancer cell proliferation and sensitivity to EGFR inhibitors. Global transcriptomics, proteomics and target prediction resulted in the identification of several tumor suppressors involved in the G1/S transition as targets of AAGUGC-microRNAs. The clinical implications of our findings were evaluated by analysis of public domain data supporting the link between this microRNA seed-family, their tumor suppressor targets and cancer cell proliferation. In conclusion we propose that AAGUGC-microRNAs are an integral part of an oncogenic signaling network, and that these findings have potential therapeutic implications, especially in selecting patients for EGFR-targeting therapy.

OTHER RELATED OMICS DATASETS IN: PXD004163PRJNA321508

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Janne Lehti�

PROVIDER: MSV000080829 | MassIVE | Fri Mar 31 14:46:00 BST 2017

SECONDARY ACCESSION(S): PXD004163

REPOSITORIES: MassIVE

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microRNAs with AAGUGC seed motif constitute an integral part of an oncogenic signaling network.

Zhou Y Y Frings O O Branca R M RM Boekel J J le Sage C C Fredlund E E Agami R R Orre L M LM

Oncogene 20160801 6

microRNA (miRNA) dysregulation is a common feature of cancer cells, but the complex roles of miRNAs in cancer are not fully elucidated. Here, we used functional genomics to identify oncogenic miRNAs in non-small cell lung cancer and evaluate their impact on response to epidermal growth factor (EGFR)-targeting therapy. Our data demonstrate that miRNAs with an AAGUGC motif in their seed sequence increase both cancer cell proliferation and sensitivity to EGFR inhibitors. Global transcriptomics, pro ...[more]

PMID: 27477696

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