Dataset Information

Time-resolved Proteome and Metabolome Profiling of Normal Lung Development

ABSTRACT: Proteomics data from mouse lung; performing unbiased protein and metabolite profiling for normal lung development

INSTRUMENT(S): Orbitrap Fusion, 6140 Quadrupole LC/MS

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Charles K. Ansong

PROVIDER: MSV000081190 | MassIVE | Thu Jun 22 22:24:00 BST 2017

SECONDARY ACCESSION(S): PXD006784

REPOSITORIES: MassIVE

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Publications

Time-resolved proteome profiling of normal lung development.

Moghieb Ahmed A Clair Geremy G Mitchell Hugh D HD Kitzmiller Joseph J Zink Erika M EM Kim Young-Mo YM Petyuk Vladislav V Shukla Anil A Moore Ronald J RJ Metz Thomas O TO Carson James J McDermott Jason E JE Corley Richard A RA Whitsett Jeffrey A JA Ansong Charles C

American journal of physiology. Lung cellular and molecular physiology 20180308 1

Biochemical networks mediating normal lung morphogenesis and function have important implications for ameliorating morbidity and mortality in premature infants. Although several transcript-level studies have examined normal lung development, corresponding protein-level analyses are lacking. Here we performed proteomics analysis of murine lungs from embryonic to early adult ages to identify the molecular networks mediating normal lung development. We identified 8,932 proteins, providing a deep an ...[more]

PMID: 29516783

Similar Datasets

Project description:Current understanding of heat shock response has been complicated by the fact that heat stress is inevitably accompanied by changes in specific growth rates and growth stages. In this study, a chemostat culture was successfully performed to avoid the physico-chemical and biological changes that accompany heatshock, which provided a unique opportunity to investigate the full range of cellular responses to thermal stress, ranging from temporary adjustment to phenotypic adaptation at multi-omics levels. Heat-responsive and time-resolved changes in the transcriptome and metabolome of a widely used E. coli strain BL21(DE3) were explored in which the temperature was upshifted from 37 to 42 °C. Omics profiles were categorized into early (2 and 10 min), middle (0.5, 1, and 2 h), and late (4, 8, and 40 h) stages of heat stress, each of which reflected the initiation, adaptation, and phenotypic plasticity steps of the stress response. The continued heat stress modulated global gene expression by controlling the expression levels of sigma factors in different time frames, including unexpected downregulation of the second heatshock sigma factor gene (rpoE) upon the heat stress. Trehalose, cadaverine, and enterobactin showed increased production to deal with the heat-induced oxidative stress. Genes highly expressed at the late stage were experimentally validated to provide thermotolerance. Intriguingly, a cryptic capsular gene cluster showed considerably high expression level only at the late stage, and its expression was essential for cell growth at high temperature. Granule-forming and elongated cells were observed at the late stage, which was morphological plasticity occurred as a result of acclimation to the continued heat stress. Whole process of thermal adaptation along with the genetic and metabolic changes at fine temporal resolution will contribute to far-reaching comprehension of the heat shock response. Further, the identified thermotolerant genes will be useful to rationally engineer thermotolerant microorganisms.