Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

AP-MS analysis using HEK293T cells transiently expressing HDAC1-Halo

ABSTRACT: AP-MS analysis using HEK293T cells transiently expressing HDAC1-Halo

INSTRUMENT(S): LTQ

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Mike Washburn

PROVIDER: MSV000081356 | MassIVE | Tue Jul 18 14:32:00 BST 2017

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

AP-MS analysis using HEK293T cells transiently expressing Halo-HDAC1

Project description:AP-MS analysis using HEK293T cells transiently expressing Halo-HDAC1

2017-07-19 | MSV000081363 | MassIVE

AP-MS analysis using HEK293T cells transiently expressing Halo-SAP30

Project description:AP-MS analysis using HEK293T cells transiently expressing Halo-SAP30

2017-07-18 | MSV000081355 | MassIVE

AP-MS analysis using HEK293T cells transiently expressing Halo-SUDS3

Project description:AP-MS analysis using HEK293T cells transiently expressing Halo-SUDS3

2017-07-19 | MSV000081361 | MassIVE

AP-MS analysis using HeLa cells transfected with Halo-HDAC1

Project description:AP-MS analysis using HeLa cells transfected with Halo-HDAC1

2017-12-15 | MSV000081820 | MassIVE

AP-MS analysis using HeLa cells transfected with HDAC1-Halo plasmid

Project description:AP-MS analysis using HeLa cells transfected with HDAC1-Halo plasmid

2017-12-15 | MSV000081821 | MassIVE

HEK293T cell proteome remodeling upon Torin1 treatment or amino acid withdraw in WT and RPS3-Halo knock-in cells with or without NUFIP1 deletion. Related to Supplementary Table 5 from An et al (2020)

Project description:HEK293T proteome (single shot) with knock-in or not for RPS3-Halo and Knock out or not for NUFIP1. Cells were treated or not with Torin1 or deprived of amino acids.

2020-04-30 | PXD017851 | Pride

Analysis of transcriptome of mouse melanoma cells co-cultured with HEK293T cells expressing mouse Siglec1.

Project description:To evaluate the effect of mouse Siglec1 on B16F10 melanoma we used co-culture methos where HEK293T cells were transiently transfected with plasmid containg mouse Siglec1 or empty plasmid (mock). These HEK293T cells were cultured with B16F10-GFP mouse melanoma cells for 18 hours in low binding plates. After 18 hours of incubation on 37°C, 5% CO2, melanoma cells were FACS sorted based on GFP expression and total RNA was isolated and used for TruSeq RNA library construction for transcriptome analysis. Samples were from 3 independent experiments. The library preparation and NGS data aquisition were performed by Macrogen (Macrogen, Inc., Korea)

2019-12-22 | GSE109077 | GEO

Overexpression of USF1 in HEK293T cells

Project description:Overexpression of USF1 in HEK293T cells in vitro to ascertain the genes downstream of USF1. Will identify direct targets as well as indirect targets of USF1. Keywords: Overexpression of transcription factor to determine downstream targets. Cloned USF1 from cDNA and transiently transfected into HEK293T cells. Total RNA collected 48 hours after transfection. Empty vector used as control. Expression of USF1 was validated using western blot, and qRT-PCR was used to validate differential expression.

2009-09-14 | E-GEOD-17300 | biostudies-arrayexpress

Analysis of subcellular transcriptomes by RNA proximity labeling with Halo-seq

Project description:We have developed Halo-seq, an RNA proximity labeling method that allows the quantification of subcellular transcriptomes. We have demonstrated the efficacy of Halo-seq here by using it to quantify chromatin-proximal, nucleolar, and cytoplasmic transcriptomes. In Halo-seq, RNA molecules in close proximity to a spatially restricted protein are specifically marked and biotinylated, facilitating their separation from bulk cellular RNA and their quantification.

2021-04-19 | GSE172281 | GEO

AP-MS analysis using Flp-In-293 cells stably expressing HDAC1 with C-terminal HaloTag

Project description:AP-MS analysis using Flp-In-293 cells stably expressing HDAC1 with C-terminal HaloTag

2020-03-27 | MSV000085176 | MassIVE