Proteomics

Dataset Information

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Youn_et_al_RNAbodies_SAINT3105_Set1


ABSTRACT: This set of submissions contains the mass spectrometry files for the manuscript by Ji-Young Youn et al. that describes the high-density proximity mapping of RNA bodies. BioID experiments were performed from Flp-In T-REx HEK293 cells and MS files were acquired on Orbitrap Elite and Orbitrap Velos.

INSTRUMENT(S): LTQ Orbitrap Velos, LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Anne-Claude Gingras  

PROVIDER: MSV000081411 | MassIVE | Fri Aug 04 11:18:00 BST 2017

SECONDARY ACCESSION(S): PXD007199

REPOSITORIES: MassIVE

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Publications


mRNA processing, transport, translation, and ultimately degradation involve a series of dedicated protein complexes that often assemble into large membraneless structures such as stress granules (SGs) and processing bodies (PBs). Here, systematic in vivo proximity-dependent biotinylation (BioID) analysis of 119 human proteins associated with different aspects of mRNA biology uncovers 7424 unique proximity interactions with 1,792 proteins. Classical bait-prey analysis reveals connections of hundr  ...[more]

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