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Characterization of indole-3-acetic acid biosynthesis and the effects of IAA exposure on the proteome of the plant-associated microbe Pantoea sp. YR343

ABSTRACT: Pantoea sp. YR343, isolated from the Populus deltoides rhizosphere, is a robust plant root colonizer that produces indole-3-acetic acid (IAA). Genomic and metabolomic analyses predicted that Pantoea sp. YR343 synthesizes IAA primarily using the indole-3-pyruvate pathway. Pantoea sp. YR343 proteomes showed upregulation of IpdC for growth in the presence of tryptophan or IAA versus controls.

INSTRUMENT(S): LTQ XL

ORGANISM(S): Pantoea Sp. Yr343 (ncbitaxon:1144341)

SUBMITTER: Jennifer L. Morrell-Falvey

PROVIDER: MSV000081478 | MassIVE | Wed Aug 23 15:26:00 BST 2017

SECONDARY ACCESSION(S): PXD007541

REPOSITORIES: MassIVE

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Publications

Characterization of Indole-3-acetic Acid Biosynthesis and the Effects of This Phytohormone on the Proteome of the Plant-Associated Microbe Pantoea sp. YR343.

Estenson Kasey K Hurst Gregory B GB Standaert Robert F RF Bible Amber N AN Garcia David D Chourey Karuna K Doktycz Mitchel J MJ Morrell-Falvey Jennifer L JL

Journal of proteome research 20180226 4

Indole-3-acetic acid (IAA) plays a central role in plant growth and development, and many plant-associated microbes produce IAA using tryptophan as the precursor. Using genomic analyses, we predicted that Pantoea sp. YR343, a microbe isolated from Populus deltoides, synthesizes IAA using the indole-3-pyruvate (IPA) pathway. To better understand IAA biosynthesis and the effects of IAA exposure on cell physiology, we characterized proteomes of Pantoea sp. YR343 grown in the presence of tryptophan ...[more]

PMID: 29464956

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