Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Elite, Q Exactive
ORGANISM(S): Saccharomycetales (ncbitaxon:4892) Homo Sapiens (ncbitaxon:9606)
SUBMITTER: Nikolai Slavov
PROVIDER: MSV000081857 | MassIVE | Fri Dec 22 11:49:00 GMT 2017
SECONDARY ACCESSION(S): PXD008557
REPOSITORIES: MassIVE
Malioutov Dmitry D Chen Tianchi T Airoldi Edoardo E Jaffe Jacob J Budnik Bogdan B Slavov Nikolai N
Molecular & cellular proteomics : MCP 20181003 1
Many proteoforms-arising from alternative splicing, post-translational modifications (PTM), or paralogous genes-have distinct biological functions, such as histone PTM proteoforms. However, their quantification by existing bottom-up mass-spectrometry (MS) methods is undermined by peptide-specific biases. To avoid these biases, we developed and implemented a first-principles model (HI<i>quant</i>) for quantifying proteoform stoichiometries. We characterized when MS data allow inferring proteoform ...[more]