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Proteomic analysis of digestive fluids of Thermobia domestica

ABSTRACT: Digestive fluids isolated from the foregut (crop) of adult specimens of T. domestica were analysed by shotgun proteomics in order to identify the putative carbohydrate-active enzymes involved in plant cell wall digestion.

INSTRUMENT(S): maXis

ORGANISM(S): Thermobia Domestica (ncbitaxon:89055)

SUBMITTER: Simon J. McQueen-Mason

PROVIDER: MSV000081912 | MassIVE | Thu Jan 11 04:25:00 GMT 2018

SECONDARY ACCESSION(S): PXD008657

REPOSITORIES: MassIVE

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Publications

An ancient family of lytic polysaccharide monooxygenases with roles in arthropod development and biomass digestion.

Sabbadin Federico F Hemsworth Glyn R GR Ciano Luisa L Henrissat Bernard B Dupree Paul P Tryfona Theodora T Marques Rita D S RDS Sweeney Sean T ST Besser Katrin K Elias Luisa L Pesante Giovanna G Li Yi Y Dowle Adam A AA Bates Rachel R Gomez Leonardo D LD Simister Rachael R Davies Gideon J GJ Walton Paul H PH Bruce Neil C NC McQueen-Mason Simon J SJ

Nature communications 20180222 1

Thermobia domestica belongs to an ancient group of insects and has a remarkable ability to digest crystalline cellulose without microbial assistance. By investigating the digestive proteome of Thermobia, we have identified over 20 members of an uncharacterized family of lytic polysaccharide monooxygenases (LPMOs). We show that this LPMO family spans across several clades of the Tree of Life, is of ancient origin, and was recruited by early arthropods with possible roles in remodeling endogenous ...[more]

PMID: 29472725

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