Proteomics

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Cell sorted lipidomics and proteomics of the 20 month human lung

ABSTRACT: Applied liquid chromatography-tandem mass spectrometry to characterize the lipidome of major lung cell types isolated from human donors, representing the first lipidome map of any organ. We coupled this with cell type-resolved proteomics of the same samples (available at lungmap.net). Complementary proteomics analyses substantiated the functional identity of the isolated cells.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Charles Ansong  

PROVIDER: MSV000081973 | MassIVE | Thu Jan 25 10:16:00 GMT 2018

REPOSITORIES: MassIVE

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Cell type-resolved proteome analyses of the brain, heart and liver have been reported, however a similar effort on the lipidome is currently lacking. Here we applied liquid chromatography-tandem mass spectrometry to characterize the lipidome of major lung cell types isolated from human donors, representing the first lipidome map of any organ. We coupled this with cell type-resolved proteomics of the same samples (available at Lungmap.net). Complementary proteomics analyses substantiated the func  ...[more]

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