Dataset Information

Arabidopsis circadian proteomics and phosphoproteomics in Col0 and CCA1-Ox

ABSTRACT: Proteomics and phosphoproteomics analyses of circadian time course samples of Arabidopsis thaliana rosettes (22 DAS) in constant light. Two independent experiments, with global proteomics and phosphoproteomics analyses for each. Experiment I has a time point every 4h from ZT12 to ZT32, experiment 2 has a time point every 4h from ZT24 to ZT52. In each experiment, two genotypes were used - WT Col0 and the transcriptionally arrhythmic CCA1-Ox line.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Prof Andrew Millar

PROVIDER: MSV000082175 | MassIVE | Fri Mar 16 07:02:00 GMT 2018

SECONDARY ACCESSION(S): PXD009230

REPOSITORIES: MassIVE

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Publications

The Circadian Clock Gene Circuit Controls Protein and Phosphoprotein Rhythms in Arabidopsis thaliana.

Krahmer Johanna J Hindle Matthew M Perby Laura K LK Mogensen Helle K HK Nielsen Tom H TH Halliday Karen J KJ van Ooijen Gerben G Le Bihan Thierry T Millar Andrew J AJ

Molecular & cellular proteomics : MCP 20211103 1

Twenty-four-hour, circadian rhythms control many eukaryotic mRNA levels, whereas the levels of their more stable proteins are not expected to reflect the RNA rhythms, emphasizing the need to test the circadian regulation of protein abundance and modification. Here we present circadian proteomic and phosphoproteomic time series from Arabidopsis thaliana plants under constant light conditions, estimating that just 0.4% of quantified proteins but a much larger proportion of quantified phospho-sites ...[more]

PMID: 34740825

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