Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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Circadian rhythms of sense and antisense transcription in a highly polyploid crop.


ABSTRACT: Sugarcane plants were grown in soil in a 12h light/ 12h dark photoperiod and 26oC for 3 months. Then, the plants were transferred to constant light conditions and 24 h later, leaves were harvested every 4 h for 48 h. Samples were collected from 24 h in constant light to 68 h in constant light and were labelled accordingly. Two biological replicates of each time point were made, with a dye swap with the reference. The reference used was a equimolar solution of all samples.

ORGANISM(S): Saccharum hybrid cultivar (mixed)

SUBMITTER: Milton Nishiyama Jr 

PROVIDER: E-GEOD-42725 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Circadian rhythms of sense and antisense transcription in sugarcane, a highly polyploid crop.

Hotta Carlos Takeshi CT   Nishiyama Milton Yutaka MY   Souza Glaucia Mendes GM  

PloS one 20130806 8


Commercial sugarcane (Saccharum hybrid) is a highly polyploid and aneuploid grass that stores large amounts of sucrose in its stem. We have measured circadian rhythms of sense and antisense transcription in a commercial cultivar (RB855453) using a custom oligoarray with 14,521 probes that hybridize to sense transcripts (SS) and 7,380 probes that hybridize to antisense transcripts (AS).We estimated that 32% of SS probes and 22% AS probes were rhythmic. This is a higher proportion of rhythmic prob  ...[more]

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