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Mouse Placenta Proteome and Phosphoproteome

ABSTRACT: TMT based quantification of protein abundance and phosphorylation state for developing mouse placenta. Timed-pregnant CD-1 mice were obtained from Charles Rivers Labs and dissected at e7.5 to extract the ectoplacental cones (EPCs) and at e9.5 to obtain the placenta as described by Martin and Cockroft (Martin, P.; Cockroft, D. L. Culture of Postimplantation Mouse Embryos). Staging of the mouse embryos was done according to Theiler criteria (Theiler, K. The House Mouse: Development and Normal Stages from Fertilization to 4 Weeks of Age; Springer-Verlag, 1972.)

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Justin Walley

PROVIDER: MSV000082849 | MassIVE | Fri Aug 24 11:41:00 BST 2018

REPOSITORIES: MassIVE

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Publications

Comparative Analysis of the Transcriptome and Proteome during Mouse Placental Development.

Abdulghani Majd M Song Gaoyuan G Kaur Haninder H Walley Justin W JW Tuteja Geetu G

Journal of proteome research 20190415 5

The condition of the placenta is a determinant of the short- and long-term health of the mother and the fetus. However, critical processes occurring in early placental development, such as trophoblast invasion and establishment of placental metabolism, remain poorly understood. To gain a better understanding of the genes involved in regulating these processes, we utilized a multiomics approach, incorporating transcriptome, proteome, and phosphoproteome data generated from mouse placental tissue ...[more]

PMID: 30986076

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