The IkB kinase complex is a regulator of mRNA stability
Ontology highlight
ABSTRACT: U2OS cells were split into two aliquots and cultured in one of
the following media (DMEM + 10 % dialysed FCS):
i) light medium: contains Arg-0 and Lys-0
ii) heavy medium: contains Arg-10 and Lys-8
Cells were passaged at least three times in the respective medium to ensure complete
labelling of proteins. SILAC experiments were performed in a forward and a reverse
reaction: for the forward experiment, heavy-labelled cells were irradiated with 20 Gy and
harvested 45 minutes after irradiation, while light-labelled cells were left untreated. In the
reverse experiment, labels were swapped and light-labelled cells were irradiated, while
heavy labelled cells were left untreated.
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
SUBMITTER: Klaus Scheidereit
PROVIDER: MSV000082975 | MassIVE |
SECONDARY ACCESSION(S): PXD011186
REPOSITORIES: MassIVE
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