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MSstats splitplot method for protein quantitation in MS datasets-SRM controlled mixture

ABSTRACT: Digested Escherichia coli protein extract were mixed with 89 different isotopically-labelled reference heavy peptides (5 pmol, 15N2,13C6-Lys, 15N4,13C6-Arg), and with different amounts of the corresponding light peptides (10 pmol, 5 pmol, 2.5 pmol), as 1, 2 and 4, respectively. Light peptides were dived in three different subsets and five different mixtures were prepared in triplicates. SRM measurements for the light-heavy synthetic peptides were performed on a hybrid triple quadrupole/ion trap mass spectrometer (4000 Q-Trap, AB Sciex) equipped with a nano-LC electrospray ionization source. Details are available in description.pdf

INSTRUMENT(S): 4000 QTRAP

ORGANISM(S): Escherichia Coli (ncbitaxon:562)

SUBMITTER: Eduard Sabido

PROVIDER: MSV000084220 | MassIVE | Wed Aug 21 13:38:00 BST 2019

SECONDARY ACCESSION(S): PXD015304

REPOSITORIES: MassIVE

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