Integrated multi-omics analysis of the NK603 Roundup-tolerant GM maize

ABSTRACT: Glyphosate tolerant genetically modified (GM) maize NK603 was assessed as substantially equivalent to its isogenic counterpart by a nutrient composition analysis in order to be granted market approval. We have applied contemporary in depth molecular profiling methods of NK603 maize kernels (sprayed or unsprayed with Roundup) and the isogenic corn to reassess its substantial equivalence status. Proteome profiles of the maize kernels revealed alterations in the levels of enzymes of glycolysis and TCA cycle pathways, which were reflective of an imbalance in energy metabolism. Changes in proteins and metabolites of glutathione metabolism were indicative of increased oxidative stress. The most pronounced metabolome differences between NK603 and its isogenic counterpart consisted of an increase in polyamines including N-acetyl-cadaverine (2.9-fold), N-acetylputrescine (1.8-fold), putrescine (2.7-fold) and cadaverine (28-fold), which depending on context can be either protective or a cause of toxicity. Our molecular profiling results show that NK603 and its isogenic control are not substantially equivalent.

INSTRUMENT(S): Orbitrap Velos-Pro mass spectrometer

ORGANISM(S): Zea Mays (ncbitaxon:4577)

SUBMITTER: Michael Antoniou  

PROVIDER: MSV000083034 | MassIVE | Mon Oct 15 09:21:00 BST 2018

SECONDARY ACCESSION(S): PXD011363

REPOSITORIES: MassIVE