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Intracellular proteome of Pyrenophora tritici-repentis isolate PtrM4

ABSTRACT: Proteome mapping of Pyrenophora tritici-repentis (Ptr) intracellular proteins Sample analysis (in brief): - Ptr samples have been concentrated via TCA/acetone protein precipitation - Concentrated samples have been separated into 24 fractions by OFFGEL electrophoresis (IEF) - Proteins of each fraction have then been reduced, alkylated (iodoacetamide) and trypsin-digested - Peptides of each fraction have then been separated on a C18 column in an LC run and analysed by high-resolution mass spectrometry (LTQ Orbitrap XL).

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Pyrenophora Tritici-repentis (ncbitaxon:45151)

SUBMITTER: Caroline Moffat

PROVIDER: MSV000083062 | MassIVE | Mon Oct 22 23:58:00 BST 2018

REPOSITORIES: MassIVE

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