Intracellular proteome of Pyrenophora tritici-repentis isolate PtrDW5
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ABSTRACT: Proteome mapping of Pyrenophora tritici-repentis (Ptr) intracellular proteins
Sample analysis (in brief): - Ptr samples have been concentrated via TCA/acetone protein precipitation - Concentrated samples have been separated into 24 fractions by OFFGEL electrophoresis (IEF) - Proteins of each fraction have then been reduced, alkylated (iodoacetamide) and trypsin-digested - Peptides of each fraction have then been separated on a C18 column in an LC run and analysed by high-resolution mass spectrometry (LTQ Orbitrap XL)
INSTRUMENT(S): LTQ Orbitrap XL
ORGANISM(S): Pyrenophora Tritici-repentis (ncbitaxon:45151)
SUBMITTER: Caroline Moffat
PROVIDER: MSV000083063 | MassIVE | Tue Oct 23 00:03:00 BST 2018
REPOSITORIES: MassIVE
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